Gonzalez, Luis MiguelCuevas, LaureanoPerez-Pastrana, EsperanzaSanta-María, YsmaelBenito, Agustin2018-11-202018-11-202009-10-09Mol Biol Rep. 2010;37(6):2893-902.0301-4851http://hdl.handle.net/20.500.12105/6634To identity immunodiagnostic antigen genes, a Plasmodium falciparum (Dd2 clone) expression library was screened using human immune sera. The ring-infected erythrocyte surface antigen (RESA) was isolated: this antigen of the resistant clone presents repeat tandem sequences like the 3D7 clone, albeit in different numbers. RESA has been studied as a marker of genetic diversity, with different sizes being observed in different isolates and clones of Plasmodium falciparum. The native protein was localised in cultures by western-blot and immuno-transmission electron microscopy. The antigenicity of RESA was evaluated by ELISA, using the carboxy-terminal repeat region as antigen. The assay's sensitivity and specificity were 78.2 and 94% respectively.engVoRhttp://creativecommons.org/licenses/by-nc/4.0/Plasmodium falciparumMalariaELISAExpression libraryElectron microscopyAmino Acid SequenceAntigens, ProtozoanBlotting, WesternCell ExtractsCloning, MolecularComputational BiologyDNA, ComplementaryGenetic MarkersHumansMolecular Sequence DataPlasmodium falciparumProtein TransportProtozoan ProteinsRecombinant Fusion ProteinsSequence AlignmentGenetic VariationAtribución-NoComercial- 4.0 Internacional198167923762893-90210.1007/s11033-009-9849-z1573-4978Molecular biology reportsopen access