Genomic characterisation of respiratory syncytial virus: a novel system for whole genome sequencing and full-length G and F gene sequences

Iglesias-Caballero, MariaCamarero-Serrano, SaraVarona Fernandez, SaraiMas-Lloret, VicenteCalvo, CristinaGarcía, María LuzGarcía-Costa, JuanVázquez-Morón, SoniaMonzon-Fernandez, SaraCampoy, AlbertCuesta de la Plaza, IsabelPozo Sanchez, FranciscoCasas Flecha, Inmaculada2023-12-122023-12-122023-12Euro Surveill. 2023 Dec;28(49).http://hdl.handle.net/20.500.12105/16776To advance our understanding of respiratory syncytial virus (RSV) impact through genomic surveillance, we describe two PCR-based sequencing systems, (i) RSVAB-WGS for generic whole-genome sequencing and (ii) RSVAB-GF, which targets major viral antigens, G and F, and is used as a complement for challenging cases with low viral load. These methods monitor RSV genetic diversity to inform molecular epidemiology, vaccine effectiveness and treatment strategies, contributing also to the standardisation of surveillance in a new era of vaccines.engVoRhttp://creativecommons.org/licenses/by/4.0/Genomic characterisation of respiratory syncytial virus: a novel system for whole genome sequencing and full-length G and F gene sequencesAtribución 4.0 Internacional38062945284910.2807/1560-7917.ES.2023.28.49.23006371560-7917Euro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletinopen access