Sahuquillo-Arce, José MiguelReyes-Prieto, MarianaHernández-Cabezas, AliciaMolina-Moreno, José MiguelSaez-Nieto, Juan AntonioMarín, María Del PilarAlcoriza-Balaguer, María IsabelLahoz, AgustínSanz, JaimeGonzález-Tarancón, LolaFerrús, María LoretoMartínez-Priego, LlúciaMagraner-Martínez, AdolfoLópez-Hontangas, José Luis2024-02-092024-02-092023-08Int J Syst Evol Microbiol. 2023 Aug;73(8).http://hdl.handle.net/20.500.12105/17697Strain HF14-78462T is an environmental bacterium found in clinical samples from an immunocompromized patient in 2014 at Hospital Universitari i Politècnic La Fe (Valencia, Spain). Phenotypically, strain HF14-78462T cells were Gram-stain-negative, aerobic, non-spore forming and non-motile small rods which formed mucous and whitish-translucent colonies when incubated at 20-36 °C. Phylogenetic analyses based on the 16S rRNA genes and the whole genomes of closest sequenced relatives confirmed that strain HF14-78462T is affiliated with the genus Starkeya. The strain was oxidase, catalase and urease positive; but indole, lysine decarboxylase, ornithine decarboxylase and DNase negative, did not produce H2S and was able to utilize a wide variety of carbon sources including acetamide, adonitol, amygdalin, l-arabinose, citric acid, glucose, mannitol and melibiose. Unlike Starkeya novella and Starkeya koreensis, strain HF14-78462T failed to grow in thiosulphate-oxidizing media and had a narrower temperature growth range. Its genome was characterized by a size of 4.83 Mbp and a C+G content of 67.75 mol%. Major fatty acids were C18:1 ω7c, cyclo C19 : 0 and C16 : 0, its polar acids were diphosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol and an aminophospholipid; while the ubiquinones were Q9 (1.8 %) and Q10 (98.2 %). Digital DNA-DNA hybridization values were 41 and 41.4 against S. novella and S. koreensis, respectively, while average nucleotide identity values were around 84 %. Phenotypic, average nucleotide identity and phylogenomic comparative studies suggest that strain HF14-78462T is a new representative of the genus Starkeya and the name Starkeya nomas sp. nov. is proposed. The type strain is HF14-78462T (=CECT 30124T=LMG 31874T).engVoREmerging pathogensImmunocompromized patientStarkeyaFatty AcidsNomaHumansPhylogenyRNA, Ribosomal, 16SSequence Analysis, DNADNA, BacterialBacterial Typing TechniquesBase CompositionBacteriaAttribution-NonCommercial-NoDerivatives 4.0 Internacional3760599873800580510.1099/ijsem.0.0058051466-5034International journal of systematic and evolutionary microbiologyopen access