Calero-Bernal, RafaelSantín, MónicaMaloney, Jenny GMartín-Pérez, ManuelHabela, Miguel ÁngelFernández-García, José LFigueiredo, AnaNájera, FernandoPalacios, María JMateo, MartaBalseiro, AnaBarral, MartaLima-Barberoi, José FKöster, Pamela CarolinaCarmena, David2020-11-112020-11-112020-03-01J Eukaryot Microbiol. 2020 Mar;67(2):273-278.1550-7408http://hdl.handle.net/20.500.12105/11317The occurrence and molecular diversity of the stramenopile eukaryote Blastocystis sp. was investigated by PCR and sequencing (Sanger and NGS) methods in 380 faecal specimens of free-living carnivores in Spain. Blastocystis sp. was confirmed in 1.6% (6/380) of the specimens analysed. Two samples from a common genet and a fox were successfully subtyped as ST7 by Sanger. Using NGS, ST14 was found in a fox and a European polecat, ST7 in a fox, and two additional foxes presented mixed infections of ST1/ST2/ST4 and ST1/ST2/ST7, respectively. Wild carnivore species could act as carriers of zoonotic Blastocystis subtypes.engAMhttp://creativecommons.org/licenses/by-nc-sa/4.0/SubtypesZoonotic transmissionSangerNext Generation SequencingWildlifeAtribución-NoComercial-CompartirIgual 4.0 Internacional31691450672273-27810.1111/jeu.12772The Journal of eukaryotic microbiologyopen access