Cogliati, SaraHerranz, FernandoRuiz-Cabello, JesúsEnriquez, Jose Antonio2022-07-072022-07-072021-01-01Biochim Biophys Acta Bioenerg. 2021;1862(1):148332http://hdl.handle.net/20.500.12105/14681The BlueNative page (BNGE) gel has been the reference technique for studying the electron transport chain organization since it was established 20 years ago. Although the migration of supercomplexes has been demonstrated being real, there are still several concerns about its ability to reveal genuine interactions between respiratory complexes. Moreover, the use of different solubilization conditions generates conflicting interpretations. Here, we thoroughly compare the impact of different digitonin concentrations on the liquid dispersions' physical properties and correlate with the respiratory complexes' migration pattern and supercomplexes. Our results demonstrate that digitonin concentration generates liquid dispersions with specific size and variability critical to distinguish between a real association of complexes from being trapped in the same micelle.engVoRhttp://creativecommons.org/licenses/by-nc-nd/4.0/Blue Native ElectrophoresisRespiratory ComplexesSupercomplexesDigitonineMitochondriaNative Polyacrylamide Gel ElectrophoresisAnimalsDigitoninElectron Transport Complex IMiceMitochondria, HeartMitochondria, LiverMitochondrial ProteinsAttribution-NonCommercial-NoDerivatives 4.0 Internacional331298271862114833210.1016/j.bbabio.2020.1483321879-2650Biochimica et biophysica acta. Bioenergeticsopen access