Guerra, CRoncero, CPorras, AFernández, MBenito, M2024-09-162024-09-161996-01-26J Biol Chem . 1996 ;271(4):2076-81.0021-9258https://hdl.handle.net/20.500.12105/23075Confluent fetal rat brown adipocytes in primary culture showed an almost undetectable level of uncoupling protein (UCP) mRNA and a low mitochondrial content of functional UCP. Treatment of confluent cells with 10 nM triiodothyronine in a serum-free medium, in the absence of noradrenergic stimulation, increased the amount of UCP mRNA in a time-dependent manner. This effect was due to an increased UCP gene transcription rate and UCP mRNA stabilization, resulting in a higher content of immunoreactive mitochondrial UCP and functional UCP (detected by its ability to bind GDP). Thus, triiodothyronine might play a significant physiological role in the UCP expression throughout fetal development, when brown adipose tissue starts to differentiate and UCP is primarily expressed.engVoRhttp://creativecommons.org/licenses/by-nc-nd/4.0/Adipose Tissue, BrownAnimalsCarrier ProteinsCells, CulturedCycloheximideGene Expression RegulationGuanosine DiphosphateIon ChannelsMembrane ProteinsMitochondriaMitochondrial ProteinsNorepinephrineProtein Synthesis InhibitorsRNA, MessengerRatsTranscription, GeneticTriiodothyronineUncoupling Protein 1Attribution-NonCommercial-NoDerivatives 4.0 Internacional85676622714207610.1074/jbc.271.4.2076The Journal of biological chemistryopen access