Espada, JesúsPeinado, HectorLopez-Serra, LidiaSetién, FernandoLopez-Serra, PaulaPortela, AnnaRenart, JaimeCarrasco, ElisaCalvo, MaríaJuarranz, AngelesCano, AmparoEsteller, Manel2024-02-122024-02-122011-11Nucleic Acids Res . 2011;39(21):9194-205http://hdl.handle.net/20.500.12105/17968Mammalian DNA methyltransferase 1 (DNMT1) is essential for maintaining DNA methylation patterns after cell division. Disruption of DNMT1 catalytic activity results in whole genome cytosine demethylation of CpG dinucleotides, promoting severe dysfunctions in somatic cells and during embryonic development. While these observations indicate that DNMT1-dependent DNA methylation is required for proper cell function, the possibility that DNMT1 has a role independent of its catalytic activity is a matter of controversy. Here, we provide evidence that DNMT1 can support cell functions that do not require the C-terminal catalytic domain. We report that PCNA and DMAP1 domains in the N-terminal region of DNMT1 are sufficient to modulate E-cadherin expression in the absence of noticeable changes in DNA methylation patterns in the gene promoters involved. Changes in E-cadherin expression are directly associated with regulation of β-catenin-dependent transcription. Present evidence suggests that the DNMT1 acts on E-cadherin expression through its direct interaction with the E-cadherin transcriptional repressor SNAIL1.engVoRhttp://creativecommons.org/licenses/by-nc-nd/4.0/Active Transport, Cell NucleusCadherinsCell AdhesionCell Line, TumorCell NucleusDNA (Cytosine-5-)-Methyltransferase 1DNA (Cytosine-5-)-MethyltransferasesDNA MethylationDown-RegulationHumansPromoter Regions, GeneticProtein Structure, TertiaryRNA, MessengerSequence DeletionSnail Family Transcription FactorsTranscription FactorsTranscription, Geneticbeta CateninAttribution-NonCommercial-NoDerivatives 4.0 Internacional218467733921919410.1093/nar/gkr6581362-4962Nucleic acids researchhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3241660/pdf/gkr658.pdfopen access