Wasniewski, SamanthaKfouri Da Silva, RafaellaCapdeville, SofíaRivera Molina, IsabelVirosta, ElenaOrtiz Cortés, CarolinaDíez-Villanueva, PabloAdeba, AntonioRuiz Lera, MartaMuñoz, LucíaAlonso, DavidGarcía, EvaEcheverría, RuthTarifa, RocíoFerrarini, AlessiaPagán, JosuéAyala, Jose LuisSolis, JorgeWood, MalissaMiranda, BlancaRodríguez Padial, LuisFernández-Friera, Leticia2025-08-062025-08-062025Front Cardiovasc Med. 2025 Mar 27:12:1535827.https://hdl.handle.net/20.500.12105/26849Cardiovascular (CV) disease is the leading cause of death in women. Although 80% of CV disease events can be prevented, mortality is projected to increase, particularly in young women. Objectives: To promote CV health in women and encourage appropriate lifestyle changes by increasing awareness through vascular ultrasound imaging. WAKE UP is a prospective case-control study in a target population of 720 asymptomatic women, aged 40-70 years with >= 1 major CV risk factor (RF). Participants will attend a baseline visit and follow-up visits at 6 and 12-months. Each visit will include the assessment of traditional and non-traditional risk factors (age, blood pressure, weight, smoking, diet, physical activity, psychosocial aspects, reproductive factors, family lifestyle), CV risk scores [Fuster-BEWAT Score (FBS) and SCORE], perception of CV disease risk, blood sampling of hormones, lipids, glycemic metabolism, inflammation parameters and omics. At baseline visit, women will be randomized to undergo 2D/3D/strain vascular ultrasound (360 with imaging vs. 360 age- and RF-matched controls without imaging). Main outcomes will include changes from baseline to follow-up in overall knowledge, attitudes, and FBS. WAKE UP trial aims to raise awareness about womens CV disease and promote lifestyle changes. Imaging can play a key role by revealing the presence of atherosclerotic plaques in a directly relatable way and thus, larger effects are anticipated in women with plaques. WAKE UP can significantly impact CV prevention by involving innovative actions addressing a major public health need and by fostering complementary and synergistic actions.The author(s) declare that financial support was received for the research and/or publication of this article. LF-F has received funding from the Instituto de Salud Carlos III, Spain (PI15/ 02019) and from Comunidad de Madrid (AORTASANA-CM; B2017/BMD-3676), Fondo Social Europeo (FSE) and Mapfre Foundation. LF-F, SW and IRM have received funding from the Instituto de Salud Carlos III, Spain (PI20/01238) and LF-F, SW from Sociedad Española de Cardiología (SEC/FEC-INV-CLI 22/05). The CNIC is supported by the ISCIII, the MICIU and the Pro CNIC Foundation, and is a Severo Ochoa Center of Excellence (grant CEX2020-001041-S funded by MICIU/AEI/10.13039/501100011033). WAKE UP study is supported by Sociedad Española de Cardiología and Daichii-Sankyo.engVoRhttp://creativecommons.org/licenses/by/4.0/cardiovascular disease preventioncardiovascular risklifestylevascular ultrasoundwomen's healthAttribution 4.0 International40212881Frontiers in Cardiovascular Medicineopen access