Nicolás-Ávila, José ÁngelSanchez-Diaz, MariaHidalgo, Andres2021-03-302021-03-302021-01-26STAR Protoc. 2021; 2(1):1002862666-1667http://hdl.handle.net/20.500.12105/12478Cardiac exophers are membrane-bound extracellular vesicles released by cardiomyocytes with varied content and an average diameter of 3.5 μm. Here, we provide a detailed protocol to enable the identification and purification of cardiomyocyte-derived exophers by using fluorescence-activated cell sorting for downstream cellular and molecular analysis. This protocol requires the use of mouse strains expressing fluorescent proteins in cardiomyocytes.engVoRhttp://creativecommons.org/licenses/by-nc-nd/4.0/Attribution-NonCommercial-NoDerivatives 4.0 Internacional334909912110028610.1016/j.xpro.2020.100286STAR Protocolsopen access