García-Fuentes, EduardoSantiago-Fernández, ConcepciónGutiérrez-Repiso, CarolinaMayas, María DOliva-Olivera, WilfredoCoín-Aragüez, LeticiaAlcaide, JuanOcaña-Wilhelmi, LuisVendrell, JoanTinahones, Francisco JGarrido-Sánchez, Lourdes2024-01-152024-01-152015-11-30http://hdl.handle.net/10668/2449http://hdl.handle.net/20.500.12105/17106BACKGROUND: A key role for HIF-1α in the promotion and maintenance of dietary obesity has been proposed. We analyzed the association between hypoxia and de novo lipogenesis in human adipose tissue. METHODS: We studied HIF-1α mRNA and protein expression in fasting status in visceral adipose tissue (VAT) from non-obese and morbidly obese subjects, and in VAT from wild-type and ob/ob C57BL6J mice in both fasting and feeding status. We also analyzed the effect of hypoxia on the VAT mRNA expression of genes involved in lipogenesis. RESULTS: HIF-1α was increased in VAT from morbidly obese subjects. In fasting status, C57BL6J ob/ob mice had a higher VAT HIF-1α mRNA expression than C57BL6J wild-type mice. In feeding status, VAT HIF-1α mRNA expression significantly increased in C57BL6J wild-type, but not in C57BL6J ob/ob mice. In humans, HIF-1α mRNA expression correlated positively with body mass index and insulin resistance. VAT HIF-1α mRNA expression correlated negatively with ACC1, PDHB and SIRT3 mRNA expression, and positively with PPAR-γ. VAT explants incubated in hypoxia showed reduced SIRT3 and increased PPAR-γ, SREBP-1c, ACLY, ACC1 and FASN mRNA expression. CONCLUSIONS: Morbidly obese subjects have a higher level of VAT HIF-1α. Postprandial status is associated with an increase in HIF-1α mRNA expression in C57BL6J wild-type mice. Hypoxia alters the mRNA expression of genes involved in de novo lipogenesis in human VAT.engVoRhttps://creativecommons.org/licenses/by/4.0/AdipogénesisTejido adiposoÍndice de masa corporalDietaAyunoFactor 1 inducible por hipoxiaResistencia a la insulinaGrasa intraabdominalLipogénesisRatonesHumanosObesidad mórbidaPPAR gammaARN mensajeroProteína 1 de unión a elementos reguladores de esterolesAdipogenesisAdipose TissueBody Mass IndexDietFastingInsulin ResistanceIntra-Abdominal FatLipogenesisMiceHumansObesity, MorbidPPAR gammaRNA, MessengerSterol Regulatory Element Binding ProteinsAttribution 4.0 International2661990710.1186/s12967-015-0732-51479-5876Journal of Translational Medicineopen access