Lozano-Prieto, MartaJorge, InmaculadaCamafeita, EmilioDevesa, Cristina ABarrero-Rodríguez, RafaelCalvo, EnriqueLaguillo-Gómez, AndreaPertusa, ClaraMartin-Cofreces, Noa BVázquez, JesúsSánchez-Madrid, Francisco2025-12-152025-12-152025-12Eur J Cell Biol. 2025 Dec;104(4):151521.https://hdl.handle.net/20.500.12105/27022The shift from quiescent to effector T cells (TC) is controlled at the translational level. Post-translational modifications (PTMs) are key factors in the diversification of protein function. Advancements in mass spectrometry-based proteomics enable proteome-wide, hypothesis-free quantitative PTM analysis. Current research highlights the centrosome role in TC activation. Here the diversity of PTMs in the TC centrosome is studied by analyzing centrosome-enriched fractions from human resting and activated T lymphoblasts. Our results show that oxidative modifications predominate in this organelle, with tryptophan as the most frequently oxidized residue. These PTMs are enriched in proteins involved in translation, vesicular trafficking, cytoskeleton organization, among others. We also demonstrate the existence of PTM changes on specific protein regions during TC activation in Myh9 (hyperoxidized), and Gzma (hypoxidized). These hyper- or hypoxidized proteins display distinct functional distributions. Our study provides the first comprehensive PTM mapping of the TC centrosome, underscoring the PTM regulatory role in TC activation.This study was supported by the Spanish Ministry of Science and Innovation, Agencia Estatal de Investigacion ´ by competitive grants PID2023–149541OB-I00, PID2022–141890B-I00, PID2020–120412RBI00, PDC2021–121797-I00 and PID2021–122348NB-I00 funded by MICIU/AEI/ 10.13039/501100011033 and by “ERDF A way of making Europe”, PLEC2022–009298, PLEC2022–009235 and EQC2021–007053-P funded by MICIU/AEI/10.13039/501100011033 and by “European Union NextGenerationEU/ PRTR”, and S2022/BMD7333-CM (INMUNOVAR-CM) and P2022/BMD7209 (INTEGRAMUNE) funded by Comunidad de Madrid. CIBER Cardiovascular (CB16/11/00272, CB16/11/00277) Fondo de Investigacion ´ Sanitaria del Instituto de Salud Carlos III; co-funding by Fondo Europeo de Desarrollo Regional (FEDER). The project leading to these results has received funding from “La Caixa” Foundation under the project codes LCF/PR/HR22/52420019 and LCF/PR/HR23/52430018. MLP is supported by a FPI fellowship (PRE2021–097478). ALG is supported by a FPU fellowship (FPU18/03882). RBR is supported by a FPU fellowship (FPU20/03365). CAD is supported by a FPI fellowship (PRE2019–090019). The CNIC is supported by the Instituto de Salud Carlos III (ISCIII), the Ministerio de Ciencia, Innovacion ´ Y Universidades (MICIU) and the Pro CNIC Foundation), and is a Severo Ochoa Center of Excellence (grant CEX2020–001041-S funded by MICIU/AEI/10.13039/501100011033).engVoRhttp://creativecommons.org/licenses/by/4.0/CentrosomePost-translational modificationsProteomicsT lymphocytesAttribution 4.0 International41046742EUROPEAN JOURNAL OF CELL BIOLOGYopen access