Ribot, JoanArreguin, AndreaKuda, OndrejKopecky, JanPalou, AndreuLuisa Bonet, Maria2024-09-102024-09-102019-08Ribot J, Arreguin A, Kuda O, Kopecky J, Palou Oliver A, Bonet ML. Novel Markers of the Metabolic Impact of Exogenous Retinoic Acid with A Focus on Acylcarnitines and Amino Acids. Int J Mol Sci. 2019 Aug;20(15):3640.http://hdl.handle.net/20.500.13003/15587https://hdl.handle.net/20.500.12105/22750Treatment with all-trans retinoic acid (ATRA), the carboxylic form of vitamin A, lowers body weight in rodents by promoting oxidative metabolism in multiple tissues including white and brown adipose tissues. We aimed to identify novel markers of the metabolic impact of ATRA through targeted blood metabolomics analyses, with a focus on acylcarnitines and amino acids. Blood was obtained from mice treated with a high ATRA dose (50 mg/kg body weight/day, subcutaneous injection) or placebo (controls) during the 4 days preceding collection. LC-MS/MS analyses with a focus on acylcarnitines and amino acids were conducted on plasma and PBMC. Main results showed that, relative to controls, ATRA-treated mice had in plasma: increased levels of carnitine, acetylcarnitine, and longer acylcarnitine species; decreased levels of citrulline, and increased global arginine bioavailability ratio for nitric oxide synthesis; increased levels of creatine, taurine and docosahexaenoic acid; and a decreased n-6/n-3 polyunsaturated fatty acids ratio. While some of these features likely reflect the stimulation of lipid mobilization and oxidation promoted by ATRA treatment systemically, other may also play a causal role underlying ATRA actions. The results connect ATRA to specific nutrition-modulated biochemical pathways, and suggest novel mechanisms of action of vitamin A-derived retinoic acid on metabolic health.enghttp://creativecommons.org/licenses/by/4.0/AcylcarnitinesAmino acidsTargeted metabolomicsRetinoic acidVitamin AAdipose TissueModels, BiologicalAmino AcidsLeukocytes, MononuclearCarnitineLipidsMaleMetabolomeMetabolomicsGene Expression ProfilingAnimalsOxidation-ReductionLipid MetabolismMiceTretinoinresearch articleAttribution 4.0 International313496132015364010.3390/ijms201536401422-0067International Journal of Molecular Sciencesopen accessAnimalesTretinoinaLípidosMasculinoTejido AdiposoCarnitinaAminoácidosLeucocitos MononuclearesModelos BiológicosMetabolismo de los LípidosRatonesMetabolomaPerfilación de la Expresión GénicaOxidación-ReducciónMetabolímica2-s2.0-85070686257482383000033L2002387189