Palacín, ArantxaGómez-Casado, CristinaRivas, Luis AAguirre, JacoboTordesillas, LeticiaBartra, JoanCarrillo, TeresaCuesta-Herranz, JavierFrutos, Consolación deGarcía Alvarez-Eire, GenovevaFernández, Francisco JGamboa, PedroMuñoz, RosaSánchez-Monge, RosaSirvent, SofíaTorres, María JVarela-Losada, SusanaRodríguez, RosalíaParro, VictorBlanca, MiguelSalcedo, GabrielDíaz-Perales, Araceli2024-01-152024-01-152012-12-14http://hdl.handle.net/10668/1212http://hdl.handle.net/20.500.12105/17044The study of cross-reactivity in allergy is key to both understanding. the allergic response of many patients and providing them with a rational treatment In the present study, protein microarrays and a co-sensitization graph approach were used in conjunction with an allergen microarray immunoassay. This enabled us to include a wide number of proteins and a large number of patients, and to study sensitization profiles among members of the LTP family. Fourteen LTPs from the most frequent plant food-induced allergies in the geographical area studied were printed into a microarray specifically designed for this research. 212 patients with fruit allergy and 117 food-tolerant pollen allergic subjects were recruited from seven regions of Spain with different pollen profiles, and their sera were tested with allergen microarray. This approach has proven itself to be a good tool to study cross-reactivity between members of LTP family, and could become a useful strategy to analyze other families of allergens.engVoRhttps://creativecommons.org/licenses/by/4.0/Reacciones CruzadasEstudios multicéntricos como asuntoHipersensibilidadAnálisis de micromatricesInmunoanálisisEspañaCross ReactionsCarrier ProteinsPlant ProteinsAntigens, PlantMulticenter Studies as TopicHypersensitivityMicroarray AnalysisImmunoassayFruitPlantsSpainPollenAttribution 4.0 International2327207210.1371/journal.pone.00507991932-6203PloS oneopen access