Rodriguez, Maria JWangorsch, AndreaGomez, FranciscaSchülke, StefanTorres, Maria JVieths, StefanScheurer, StephanToda, MasakoMayorga, Cristobalina2024-02-082024-02-082018-06-13http://hdl.handle.net/10668/12711http://hdl.handle.net/20.500.12105/17608The use of hypoallergenic derivatives is considered beneficial to promote the safety and efficacy of allergen-specific immunotherapy. We aimed to assess the efficacy of reduced and alkylated (R/A) Pru p 3, a hypoallergenic folding variant of the major peach allergen, in subcutaneous immunotherapy (SCIT) using a murine model of peach allergy. After sensitization with Pru p 3, BALB/c mice received SCIT with Pru p 3 or R/A Pru p 3 and were challenged with Pru p 3. SCIT with Pru p 3, but not with R/A Pru p 3, suppressed anaphylaxis upon the challenge significantly. SCIT with Pru p 3 did not suppress Pru p 3-specific IgE and IgG1 production, but enhanced IgG2a production. In contrast, SCIT with R/A Pru p 3 suppressed IgE and IgG1 production, but enhanced IgG2a production only moderately. The therapeutic efficacy of SCIT with Pru p 3 was associated with induction of IL-10 and IFN-γ. Hypoallergenic folding variant of Pru p 3 is not likely an efficacious therapeutic component in SCIT of peach allergy. The lower efficacy of R/A Pru p 3 might be attributed to poor antigenicity and/or weak stability due to its unfolded conformation.engVoRhttp://creativecommons.org/licenses/by/4.0/AnaphylaxisAnimalsAntigens, PlantDesensitization, ImmunologicDisease Models, AnimalFemaleFood HypersensitivityImmunoglobulin EImmunoglobulin GMiceMice, Inbred BALB CPlant ProteinsPrunus persicaT-Lymphocytes, Helper-InducerT-Lymphocytes, RegulatoryTh2 CellsAttribution 4.0 International300091862018347918510.1155/2018/34791852314-7156Journal of immunology researchopen access