2026-07-22T03:36:48Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/99682024-09-27T07:55:34Zcom_20.500.12105_19604com_20.500.12105_2051col_20.500.12105_19605
Repisalud
author
Binek, Aleksandra
author
Rojo, David
author
Godzien, Joanna
author
Rupérez, Francisco Javier
author
Nunez, Vanessa
author
Jorge, Inmaculada
author
Ricote, Mercedes
author
Vazquez, Jesus
author
Barbas, Coral
funder
Ministerio de Economía y Competitividad (España)
funder
Instituto de Salud Carlos III
funder
Fundación La Marató TV3
funder
Unión Europea. Fondo Europeo de Desarrollo Regional (FEDER/ERDF)
funder
Unión Europea. Comisión Europea
funder
Fundación ProCNIC
2020-05-08T07:16:29Z
2020-05-08T07:16:29Z
2019-01
J Proteome Res. 2019; 18(1):16981
10.1021/acs.jproteome.8b00472
1535-3907
1535-3893
Journal of proteome research
30362351
http://hdl.handle.net/20.500.12105/9968
The characterization of specialized cell subpopulations in a heterogeneous tissue is essential for understanding organ function in health and disease. A popular method of cell isolation is fluorescence-activated cell sorting (FACS) based on probes that bind surface or intracellular markers. In this study, we analyze the impact of FACS on the cell metabolome of mouse peritoneal macrophages. Compared with directly pelleted macrophages, FACS-treated cells had an altered content of metabolites related to the plasma membrane, activating a mechanosensory signaling cascade causing inflammation-like stress. The procedure also triggered alterations related to energy consumption and cell damage. The observed changes mostly derive from the physical impact on cells during their passage through the instrument. These findings provide evidence of FACS-induced biochemical changes, which should be taken into account in the design of robust metabolic assays of cells separated by flow cytometry.
eng
CE−MS
GC−MS
LC−MS
Fluorescence activated cell sorting (FACS)
Metabolome profile
Metabolomics
Multiplatform analysis
Sorted cells
Sorting
Flow Cytometry Has a Significant Impact on the Cellular Metabolome
journal article
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URL
https://repisalud.isciii.es/bitstreams/55d867b3-c2d0-44e1-ad52-087197fc7730/download
File
MD5
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application/pdf
FlowCytometrySignificantImpact_2019.pdf
URL
https://repisalud.isciii.es/bitstreams/3133e227-c78d-47b5-bcde-4b6469b52c9b/download
File
MD5
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text/plain
FlowCytometrySignificantImpact_2019.pdf.txt