2026-07-22T03:36:46Zhttps://repisalud.isciii.es/rest/oai/request

oai:repisalud.isciii.es:20.500.12105/99682024-09-27T07:55:34Zcom_20.500.12105_19604com_20.500.12105_2051col_20.500.12105_19605

00925njm 22002777a 4500 dc Binek, Aleksandra author Rojo, David author Godzien, Joanna author Rupérez, Francisco Javier author Nunez, Vanessa author Jorge, Inmaculada author Ricote, Mercedes author Vazquez, Jesus author Barbas, Coral author 2019-01 The characterization of specialized cell subpopulations in a heterogeneous tissue is essential for understanding organ function in health and disease. A popular method of cell isolation is fluorescence-activated cell sorting (FACS) based on probes that bind surface or intracellular markers. In this study, we analyze the impact of FACS on the cell metabolome of mouse peritoneal macrophages. Compared with directly pelleted macrophages, FACS-treated cells had an altered content of metabolites related to the plasma membrane, activating a mechanosensory signaling cascade causing inflammation-like stress. The procedure also triggered alterations related to energy consumption and cell damage. The observed changes mostly derive from the physical impact on cells during their passage through the instrument. These findings provide evidence of FACS-induced biochemical changes, which should be taken into account in the design of robust metabolic assays of cells separated by flow cytometry. J Proteome Res. 2019; 18(1):16981 10.1021/acs.jproteome.8b00472 1535-3907 1535-3893 Journal of proteome research 30362351 http://hdl.handle.net/20.500.12105/9968 CE−MS GC−MS LC−MS Fluorescence activated cell sorting (FACS) Metabolome profile Metabolomics Multiplatform analysis Sorted cells Sorting Flow Cytometry Has a Significant Impact on the Cellular Metabolome