2026-06-14T03:32:29Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/99572024-09-27T09:25:38Zcom_20.500.12105_19604com_20.500.12105_2051col_20.500.12105_19605
Repisalud
author
Alvarez, Veronica
author
Sánchez-Margallo, Francisco Miguel
author
Macías-García, Beatriz
author
Gomez-Serrano, Maria
author
Jorge, Inmaculada
author
Vazquez, Jesus
author
Blázquez, Rebeca
author
Casado, Javier G
funder
Instituto de Salud Carlos III
funder
Unión Europea. Fondo Europeo de Desarrollo Regional (FEDER/ERDF)
funder
Government of Extremadura (España)
funder
Ministerio de Economía, Industria y Competitividad (España)
funder
Fundación La Marató TV3
funder
Ministerio de Ciencia, Innovación y Universidades (España)
funder
Fundación ProCNIC
2020-05-07T15:22:05Z
2020-05-07T15:22:05Z
2018-10
Wiley
10.1002/term.2743
1932-7005
1932-6254
Journal of tissue engineering and regenerative medicine
30058282
http://hdl.handle.net/20.500.12105/9957
Endometrial mesenchymal stem cells (endMSCs) reside in the basal and functional layer of human endometrium and participate in tissue remodelling, which is required for maintaining the regenerative capacity of the endometrium. The endMSCs are multipotent stem cells and exhibit immunomodulatory effects. This paper aimed to evaluate the regulatory effects of extracellular vesicles derived from endMSCs (EV-endMSCs) in the setting of T cell activation. In vitro stimulations of lymphocytes were performed in the presence of EV-endMSCs. These in vitro-stimulated lymphocytes were functionally and phenotypically characterized to distinguish CD4+ and CD8+ T cell differentiation subsets. Moreover, the inhibition of TGFβ was performed with neutralizing antibodies. The phenotype and nanoparticle tracking analysis of the EV-endMSCs demonstrated that they are similar in terms of size distribution to other mesenchymal stem cells-derived exosomes. The in vitro assays showed an immunomodulatory potential of these vesicles to counteract the differentiation of CD4+ T cells. The quantification of active TGFβ in EV-endMSCs was found to be very high when compared with extracellular vesicles-free concentrated supernatants. Finally, the neutralization of TGFβ significantly attenuated the immunomodulatory activity of EV-endMSCs. In summary, this is the first report demonstrating that EV-endMSCs exhibit a potent inhibitory effect against CD4+ T cell activation, which is partially mediated by TGFβ signalling.
eng
TGFβ
Endometrial mesenchymal stem cells
Extracellular vesicles
Immunomodulation
Lymphocyte activation
Lymphocyte differentiation
The immunomodulatory activity of extracellular vesicles derived from endometrial mesenchymal stem cells on CD4+ T cells is partially mediated by TGFbeta
journal article
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URL
https://repisalud.isciii.es/bitstreams/a7485b47-8f7e-47d9-9237-59e28cdbe191/download
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ImmunomodulatoryActivityExtracellularVesicles_2018.pdf
URL
https://repisalud.isciii.es/bitstreams/4bcbc7d0-a7cc-4689-a0b3-471c113384ea/download
File
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text/plain
ImmunomodulatoryActivityExtracellularVesicles_2018.pdf.txt