2026-07-16T14:21:25Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/99182025-05-05T15:05:25Zcom_20.500.12105_2052com_20.500.12105_2051com_20.500.12105_15322col_20.500.12105_19609col_20.500.12105_16958col_20.500.12105_16967col_20.500.12105_16982col_20.500.12105_16985col_20.500.12105_16988
Repisalud
author
Blasco, Lucia
author
Ambroa, Antón
author
Trastoy, Rocío
author
Bleriot, Ines
author
Moscoso, Miriam
author
Fernández-García, Laura
author
Pérez-Nadales, Elena
author
Fernández-Cuenca, Felipe
author
Torre-Cisneros, Julian
author
Oteo-Iglesias, Jesus
author
Oliver, Antonio
author
Cantón, Rafael
author
Kidd, Tim
author
Navarro, Ferran
author
Miró, Elisenda
author
Pascual, Álvaro
author
Bou, Germán
author
Martínez-Martínez, Luis
author
Tomas, Maria
funder
Instituto de Salud Carlos III
funder
Unión Europea. Fondo Europeo de Desarrollo Regional (FEDER/ERDF)
funder
RETICS-Investigación en Patología Infecciosa (REIPI-ISCIII) (España)
funder
Xunta de Galicia (España)
2020-05-06T08:33:27Z
2020-05-06T08:33:27Z
2020-04-28
Blasco L, Ambroa A, Trastoy R, Bleriot I, Moscoso M, Fernandez-Garcia L, et al. In vitro and in vivo efficacy of combinations of colistin and different endolysins against clinical strains of multi-drug resistant pathogens. Sci Rep. 2020 Apr 28;10(1):7163.
10.1038/s41598-020-64145-7
2045-2322
2045-2322
Scientific reports
http://hdl.handle.net/20.500.13003/10931
32346029
L631657427
2-s2.0-85083992764
http://hdl.handle.net/20.500.12105/9918
560713100014
The emergence of multidrug resistant (MDR) pathogenic bacteria is jeopardizing the value of antimicrobials, which had previously changed the course of medical science. In this study, we identified endolysins ElyA1 and ElyA2 (GH108-PG3 family), present in the genome of bacteriophages Ab1051Φ and Ab1052Φ, respectively. The muralytic activity of these endolysins against MDR clinical isolates (Acinetobacter baumannii, Pseudomonas aeruginosa and Klebsiella pneumoniae) was tested using the turbidity reduction assay. Minimal inhibitory concentrations (MICs) of endolysin, colistin and a combination of endolysin and colistin were determined, and the antimicrobial activity of each treatment was confirmed by time kill curves. Endolysin ElyA1 displayed activity against all 25 strains of A. baumannii and P. aeruginosa tested and against 13 out of 17 strains of K. pneumoniae. Endolysin ElyA2 did not display any such activity. The combined antimicrobial activity of colistin and ElyA1 yielded a reduction in the colistin MIC for all strains studied, except K. pneumoniae. These results were confirmed in vivo in G. mellonella survival assays and in murine skin and lung infection models. In conclusion, combining colistin (1/4 MIC) with the new endolysin ElyA1 (350 µg) enhanced the bactericidal activity of colistin in both in vitro and in vivo studies. This will potentially enable reduction of the dose of colistin used in clinical practice.
eng
In vitro and in vivo efficacy of combinations of colistin and different endolysins against clinical strains of multi-drug resistant pathogens
research article
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https://repisalud.isciii.es/bitstreams/4ba063f2-2012-4b87-a892-756b1ac50867/download
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application/pdf
InVitroAndIn_2020.pdf
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https://repisalud.isciii.es/bitstreams/2a8518a6-c4b4-445f-b5cb-54654d09214f/download
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InVitroAndIn_2020.pdf.txt