2026-08-29T22:06:57Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/99102024-09-27T20:16:46Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19609
Repisalud
author
Vazquez, Ana
author
Herrero-Romero, Laura
author
Negredo, Anabel
author
Hernandez, Lourdes
author
Sánchez-Seco, María Paz
author
Tenorio, Antonio
2020-05-06T07:17:53Z
2020-05-06T07:17:53Z
2016
J Virol Methods. 2016 Oct;236:266-270.
10.1016/j.jviromet.2016.07.026
1879-0984
0166-0934
Journal of virological methods
27481597
http://hdl.handle.net/20.500.12105/9910
West Nile virus (WNV) is one of the most widespread arbovirus and a large variety of WNV strains and lineages have been described. The molecular methods for the diagnosis of WNV target mainly lineages 1 and 2, which have caused outbreaks in humans, equines and birds. But the last few years new and putative WNV lineages of unknown pathogenicity have been described. Here we describe a new sensitive and specific real-time PCR assay for the detection and quantification of all the WNV lineages described until now. Primers and probe were designed in the 3'-untranslated region (3'-UTR) of the WNV genome and were designed to match all sequenced WNV strains perfectly. The sensitivity of the assay ranged from 1,5 to 15 copies per reaction depending on the WNV lineage tested. The method was validated for WNV diagnosis using different viral strains, human samples (cerebrospinal fluid, biopsies, serum and plasma) and mosquito pools. The assay did not amplify any other phylogenetically or symptomatically related viruses. All of the above make it a very suitable tool for the diagnosis of WNV and for surveillance studies.
eng
Diagnosis
Flavivirus
Lineages
Real-time PCR
Surveillance
West Nile virus
Real time PCR assay for detection of all known lineages of West Nile virus
journal article
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URL
https://repisalud.isciii.es/bitstreams/813dc319-6eab-4497-9b9d-654edf3b077b/download
File
MD5
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application/pdf
RealTimePCRAssay_2016.pdf
URL
https://repisalud.isciii.es/bitstreams/3d9785ff-b9af-49e6-9308-7e9ab4101366/download
File
MD5
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text/plain
RealTimePCRAssay_2016.pdf.txt