2026-07-23T17:49:33Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/98752024-09-27T08:52:25Zcom_20.500.12105_19604com_20.500.12105_2051col_20.500.12105_19605
Repisalud
author
Alonso-Herranz, Laura
author
Porcuna, Jesus
author
Ricote, Mercedes
funder
Ministerio de Economía y Competitividad (España)
funder
Fundación La Marató TV3
funder
Comunidad de Madrid (España)
funder
Fundación La Caixa
funder
Fundación ProCNIC
2020-05-05T07:41:08Z
2020-05-05T07:41:08Z
2019-03
Methods Mol Biol. 2019; 1951:59-73
10.1007/978-1-4939-9130-3_5
1940-6029
1064-3745
Methods in molecular biology (Clifton, N.J.)
30825144
http://hdl.handle.net/20.500.12105/9875
Tissue resident macrophages (TRMs) are multifunctional immune cells present in all tissues, contributing to the correct development, homeostasis, and protection against pathogens and injury. TRMs are morphologically and functionally heterogeneous, as a result of both the diversity of tissue environments in which they reside and their complex origin. Furthermore, some specific TRM populations are controlled by nuclear receptors. A widely used method for studying the role of nuclear receptors in immune cells is flow cytometry. Although flow cytometry is extensively used in tissues such as the peripheral blood, lymph nodes, peritoneal cavity, and bone marrow, there is a need for protocols for the study TRMs in solid tissues.In this chapter, we describe a comprehensive protocol for obtaining single-cell suspensions of resident macrophages from the pleural cavity, heart, lung, spleen, and kidney, and we present detailed gating strategies for the study of nuclear receptor activity in different TRM subsets within these tissues.
eng
Flow cytometry
Heart
Kidney
Lung
Nuclear receptors
Pleural cavity
Spleen
Tissue macrophages
Isolation and Purification of Tissue Resident Macrophages for the Analysis of Nuclear Receptor Activity
journal article
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