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oai:repisalud.isciii.es:20.500.12105/98132024-09-27T20:48:00Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19609

00925njm 22002777a 4500 dc Cafini, Fabio author Thi Le Thuy, Nguyen author Roman, Federico author Prieto, José author Dubrac, Sarah author Msadek, Tarek author Morikawa, Kazuya author 2017 One important feature of the major opportunistic human pathogen Staphylococcus aureus is its extraordinary ability to rapidly acquire resistance to antibiotics. Genomic studies reveal that S. aureus carries many virulence and resistance genes located in mobile genetic elements, suggesting that horizontal gene transfer (HGT) plays a critical role in S. aureus evolution. However, a full and detailed description of the methodology used to study HGT in S. aureus is still lacking, especially regarding natural transformation, which has been recently reported in this bacterium. This work describes three protocols that are useful for the in vitro investigation of HGT in S. aureus: conjugation, phage transduction, and natural transformation. To this aim, the cfr gene (chloramphenicol/florfenicol resistance), which confers the Phenicols, Lincosamides, Oxazolidinones, Pleuromutilins, and Streptogramin A (PhLOPSA)-resistance phenotype, was used. Understanding the mechanisms through which S. aureus transfers genetic materials to other strains is essential to comprehending the rapid acquisition of resistance and helps to clarify the modes of dissemination reported in surveillance programs or to further predict the spreading mode in the future. J Vis Exp. 2017 Mar 10;(121). 1940-087X http://hdl.handle.net/20.500.12105/9813 28362383 10.3791/55087 1940-087X Journal of visualized experiments : JoVE Methodology for the Study of Horizontal Gene Transfer in Staphylococcus aureus