2026-08-29T17:25:34Zhttps://repisalud.isciii.es/rest/oai/request

oai:repisalud.isciii.es:20.500.12105/98112024-09-28T00:00:01Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19609

00925njm 22002777a 4500 dc Ta Tang, Thuy-Huong author López-Vélez, Rogelio author Lanza-Suarez, Marta author Shelley, Anthony John author Rubio Muñoz, Jose Miguel author Luz, Sérgio Luiz Bessa author 2010-09 We present filaria-nested polymerase chain reaction (PCR), which is based on amplification of first internal transcribed spacer rDNA to distinguish three parasitic filarial species (Onchocerca volvulus, Mansonella ozzardi and Mansonella perstans) that can be found in the Amazon Region. Nested PCR-based identifications yielded the same results as those utilizing morphological characters. Nested PCR is highly sensitive and specific and it detects low-level infections in both humans and vectors. No cross-amplifications were observed with various other blood parasites and no false-positive results were obtained with the nested PCR. The method works efficiently with whole-blood, blood-spot and skin biopsy samples. Our method may thus be suitable for assessing the efficacy of filaria control programmes in Amazonia by recording parasite infections in both the human host and the vector. By specifically differentiating the major sympatric species of filaria, this technique could also enhance epidemiological research in the region. Instituto Oswaldo Cruz. Ministério da Saúde 10.1590/s0074-02762010000600016 1678-8060 Memorias do Instituto Oswaldo Cruz 20945000 http://hdl.handle.net/20.500.12105/9811 Nested PCR to detect and distinguish the sympatric filarial species Onchocerca volvulus, Mansonella ozzardi and Mansonella perstans in the Amazon Region