2026-06-14T05:05:42Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/96512024-10-31T11:41:13Zcom_20.500.12105_19604com_20.500.12105_2051col_20.500.12105_19605col_20.500.12105_19607
Repisalud
author
Bonzon-Kulichenko, Elena
author
Camafeita, Emilio
author
Lopez, Juan Antonio
author
Gómez-Serrano, María
author
Jorge, Inmaculada
author
Calvo, Enrique
author
Nunez, Estefania
author
Trevisan-Herraz, Marco
author
Bagwan, Navratan
author
Bárcena, José Antonio
author
Peral, Belén
author
Vazquez, Jesus
funder
Ministerio de Ciencia, Innovación y Universidades (España)
funder
Ministerio de Economía, Industria y Competitividad (España)
funder
Instituto de Salud Carlos III
funder
Unión Europea. Fondo Europeo de Desarrollo Regional (FEDER/ERDF)
funder
Fundación La Caixa
funder
Fundación La Marató TV3
funder
Fundación ProCNIC
2020-04-21T10:38:19Z
2020-04-21T10:38:19Z
2020-03
J Proteomics. 2020; 214:103624
10.1016/j.jprot.2019.103624
1876-7737
1874-3919
Journal of proteomics
31874222
http://hdl.handle.net/20.500.12105/9651
Changes in the oxidation state of protein Cys residues are involved in cell signalling and play a key role in a variety of pathophysiological states. We had previously developed GELSILOX, an in-gel method that enables the large-scale, parallel analysis of dynamic alterations to the redox state of Cys sites and protein abundance changes. Here we present FASILOX, a further development of the GELSILOX approach featuring: i) significantly increased peptide recovery, ii) enhanced sensitivity for the detection of Cys oxidative alterations, and iii) streamlined workflow that results in shortened assay duration. In mitochondria isolated from the adipose tissue of obese, diabetic patients, FASILOX revealed a sexually dimorphic trait of Cys oxidation involving mainly mitochondrial oxidative phosphorylation complexes. These results provide the first evidence for a decreased efficiency in the antioxidant response of men as compared to women.
eng
Adipose tissue
Cys oxidation
FASILOX
Filter-aided sample preparation
Sexual dimorphism
Thiol redox proteome
Improved integrative analysis of the thiol redox proteome using filter-aided sample preparation
journal article
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