2026-06-14T05:06:57Zhttps://repisalud.isciii.es/rest/oai/request

oai:repisalud.isciii.es:20.500.12105/96512024-10-31T11:41:13Zcom_20.500.12105_19604com_20.500.12105_2051col_20.500.12105_19605col_20.500.12105_19607

00925njm 22002777a 4500 dc Bonzon-Kulichenko, Elena author Camafeita, Emilio author Lopez, Juan Antonio author Gómez-Serrano, María author Jorge, Inmaculada author Calvo, Enrique author Nunez, Estefania author Trevisan-Herraz, Marco author Bagwan, Navratan author Bárcena, José Antonio author Peral, Belén author Vazquez, Jesus author 2020-03 Changes in the oxidation state of protein Cys residues are involved in cell signalling and play a key role in a variety of pathophysiological states. We had previously developed GELSILOX, an in-gel method that enables the large-scale, parallel analysis of dynamic alterations to the redox state of Cys sites and protein abundance changes. Here we present FASILOX, a further development of the GELSILOX approach featuring: i) significantly increased peptide recovery, ii) enhanced sensitivity for the detection of Cys oxidative alterations, and iii) streamlined workflow that results in shortened assay duration. In mitochondria isolated from the adipose tissue of obese, diabetic patients, FASILOX revealed a sexually dimorphic trait of Cys oxidation involving mainly mitochondrial oxidative phosphorylation complexes. These results provide the first evidence for a decreased efficiency in the antioxidant response of men as compared to women. J Proteomics. 2020; 214:103624 10.1016/j.jprot.2019.103624 1876-7737 1874-3919 Journal of proteomics 31874222 http://hdl.handle.net/20.500.12105/9651 Adipose tissue Cys oxidation FASILOX Filter-aided sample preparation Sexual dimorphism Thiol redox proteome Improved integrative analysis of the thiol redox proteome using filter-aided sample preparation