2026-06-14T04:01:34Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/96112025-05-05T12:19:09Zcom_20.500.12105_15322com_20.500.12105_2051com_20.500.12105_2052col_20.500.12105_16963col_20.500.12105_16969col_20.500.12105_19617
Repisalud
author
Garranzo-Asensio, Maria
author
San Segundo-Acosta, Pablo
author
Martínez-Useros, Javier
author
Montero-Calle, Ana Maria
author
Fernández-Aceñero, María Jesús
author
Häggmark-Månberg, Anna
author
Peláez-García, Alberto
author
Villalba, Mayte
author
Rábano, Alberto
author
Nilsson, Peter
author
Barderas Manchado, Rodrigo
funder
Ministerio de Economía y Competitividad (España)
funder
Ministerio de Educación, Cultura y Deporte (España)
2020-04-17T15:54:48Z
2020-04-17T15:54:48Z
2018-02-16
Oncotarget. 2018 Jan 24;9(13):10847-10867.
10.18632/oncotarget.24303
1949-2553
1949-2553
Oncotarget
29541381
http://hdl.handle.net/20.500.12105/9611
Alzheimer's disease (AD) is the most common form of dementia in developed countries. A better understanding of the events taking place at the molecular level would help to identify novel protein alterations, which might be used in diagnosis or for treatment development. In this study, we have performed the high-throughput analysis of 706 molecules mostly implicated in cell-cell communication and cell signaling processes by using two antibody microarray platforms. We screened three AD pathological groups -each one containing four pooled samples- from Braak stages IV, V and VI, and three control groups from two healthy subjects, five frontotemporal and two vascular dementia patients onto Panorama and L-Series antibody microarrays to identify AD-specific alterations not common to other dementias. Forty altered proteins between control and AD groups were detected, and validated by i) meta-analysis of mRNA alterations, ii) WB, and iii) FISH and IHC using an AD-specific tissue microarray containing 44 samples from AD patients at different Braak stages, and frontotemporal and vascular dementia patients and healthy individuals as controls. We identified altered proteins in AD not common to other dementias like the E3 ubiquitin-protein ligase TOPORS, Layilin and MICB, and validated the association to AD of the previously controverted proteins DDIT3 and the E3 ubiquitin-protein ligase XIAP. These altered proteins constitute interesting targets for further immunological analyses using sera, plasma and CSF to identify AD blood- or cerebrospinal fluid-biomarkers and to perform functional analysis to determine their specific role in AD, and their usefulness as potential therapeutic targets of intervention.
eng
Alzheimer’s disease
Gerotarget/Aging
neurodegeneration
protein/antibody microarrays
proteomics
Identification of prefrontal cortex protein alterations in Alzheimer's disease
research article
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URL
https://repisalud.isciii.es/bitstreams/af759c0e-2963-49e7-80ad-7e8ab00d993f/download
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IdentificationOfPrefrontalCortex_2018.pdf
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https://repisalud.isciii.es/bitstreams/ee86c2d8-74db-4b8c-b793-154544e7a197/download
File
MD5
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text/plain
IdentificationOfPrefrontalCortex_2018.pdf.txt