2026-07-21T14:26:57Zhttps://repisalud.isciii.es/rest/oai/request

oai:repisalud.isciii.es:20.500.12105/95342025-06-17T10:37:46Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19616

00925njm 22002777a 4500 dc López-Cacho, José Manuel author Gallardo, Soledad author Posada De la Paz, Manuel author Aguerri, Miriam author Calzada, David author Mayayo, Teodoro author González-Rodríguez, María Luisa author Rabasco, Antonio María author Lahoz, Carlos author Cárdaba, Blanca author 2014 This study aimed to search the correlation among immunological profiles and clinical phenotypes of scleroderma in well-characterized groups of scleroderma patients, comparing forty-nine scleroderma patients stratified according to specific clinical phenotypes with forty-nine healthy controls. Five immunological cell subpopulations (B, CD4(+) and CD8(+) T-cells, NK, and monocytes) and their respective stages of apoptosis and activation were analyzed by flow cytometry, in samples of peripheral blood mononuclear cells (PBMCs). Analyses of results were stratified according to disease stage, time since the diagnosis, and visceral damage (pulmonary fibrosis, pulmonary hypertension, and cardiac affliction) and by time of treatment with corticosteroids. An increase in the percentages of monocytes and a decrease in the B cells were mainly related to the disease progression. A general apoptosis decrease was found in all phenotypes studied, except in localized scleroderma. An increase of B and NK cells activation was found in patients diagnosed more than 10 years ago. Specific cell populations like monocytes, NK, and B cells were associated with the type of affected organ. This study shows how, in a heterogeneous disease, proper patient's stratification according to clinical phenotypes allows finding specific cellular profiles. Our data may lead to improvements in the knowledge of prognosis factors and to aid in the analysis of future specific therapies. Biomed Res Int. 2014;2014:148293. 10.1155/2014/148293 2314-6141 2314-6133 BioMed research international 24818126 http://hdl.handle.net/20.500.12105/9534 Association of immunological cell profiles with specific clinical phenotypes of scleroderma disease