2026-06-30T22:24:45Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/95312024-09-27T22:35:48Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19609
Repisalud
author
Martin-Martin, Ines
author
Molina, Ricardo
author
Jimenez, Maribel
funder
Ministerio de Ciencia e Innovación (España)
funder
Unión Europea
2020-04-13T12:37:03Z
2020-04-13T12:37:03Z
2013
Biomed Res Int. 2013;2013:526069.
10.1155/2013/526069
2314-6141
2314-6133
BioMed research international
24171166
http://hdl.handle.net/20.500.12105/9531
Sand fly salivary proteins are on the spotlight to become vaccine candidates against leishmaniasis and to markers of exposure to sand fly bites due to the host immune responses they elicit. Working with the whole salivary homogenate entails serious drawbacks such as the need for maintaining sand fly colonies and the laborious task of glands dissection. In order to overcome these difficulties, producing recombinant proteins of different vectors has become a major task. In this study, a cDNA library was constructed with the salivary glands of Phlebotomus perniciosus from Madrid, Spain, the most widespread vector of Leishmania infantum in the Mediterranean basin. Analysis of the cDNA sequences showed several polymorphisms among the previously described salivary transcripts. The apyrase SP01B and the D7-related protein SP04 were successfully cloned, expressed in Escherichia coli, and purified. Besides, recombinant proteins were recognized by sera of hamsters and mice previously immunized with saliva through the exposure to uninfected sand fly bites. These results suggest that these two recombinant proteins conserved their immunogenic properties after expression in a prokaryote system. Therefore, this work contributes to expand the knowledge of P. perniciosus saliva that would be eventually used for the development of tools for vector control programs.
eng
Molecular and immunogenic properties of apyrase SP01B and D7-related SP04 recombinant salivary proteins of Phlebotomus perniciosus from Madrid, Spain
journal article
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URL
https://repisalud.isciii.es/bitstreams/eabdda85-572f-4b28-b389-a87a31d2571c/download
File
MD5
d7a12d6958373bee1c1d92fb669a5761
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application/pdf
MolecularAndImmunogenicProperties_2013.pdf
URL
https://repisalud.isciii.es/bitstreams/542f4cd2-005d-4187-a0b3-721c4da58669/download
File
MD5
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text/plain
MolecularAndImmunogenicProperties_2013.pdf.txt