2026-06-14T03:32:23Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/94212025-05-23T13:08:32Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19609
Repisalud
author
Martin-Vicente, Maria
author
González-Riaño, Carolina
author
Barbas, Coral
author
Jimenez-Sousa, Maria Angeles
author
Brochado-Kith, Oscar
author
Resino, Salvador
author
Martinez, Isidoro
funder
Instituto de Salud Carlos III
2020-04-03T07:38:13Z
2020-04-03T07:38:13Z
2020
PLoS One. 2020 Mar 26;15(3):e0230844.
10.1371/journal.pone.0230844
1932-6203
1932-6203
PloS one
32214395
http://hdl.handle.net/20.500.12105/9421
Viral infections induce substantial metabolic changes in infected cells to optimize viral production while cells develop countermeasures to restrict that infection. Human respiratory syncytial virus (HRSV) is an infectious pathogen that causes severe lower respiratory tract infections (LRTI) in infants, the elderly, and immunocompromised adults for which no effective treatment or vaccine is currently available. In this study, variations in metabolite levels at different time points post-HRSV infection of epithelial cells were studied by untargeted metabolomics using liquid chromatography/mass spectrometry analysis of methanol cell extracts. Numerous metabolites were significantly upregulated after 18 hours post-infection, including nucleotides, amino acids, amino and nucleotide sugars, and metabolites of the central carbon pathway. In contrast, most lipid classes were downregulated. Additionally, increased levels of oxidized glutathione and polyamines were associated with oxidative stress in infected cells. These results show how HRSV infection influences cell metabolism to produce the energy and building blocks necessary for virus reproduction, suggesting potential therapeutic interventions against this virus.
eng
Metabolic changes during respiratory syncytial virus infection of epithelial cells
research article
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URL
https://repisalud.isciii.es/bitstreams/e2b29187-6231-42af-8c54-250f95bd35b2/download
File
MD5
04c5533460da616bc55ec36f43a44613
3149955
application/pdf
MetaboliChangesDuringRespiratory_2020.pdf
URL
https://repisalud.isciii.es/bitstreams/ed13a39b-9358-408c-b090-d715d2e257b8/download
File
MD5
a305dd4a8202774e11d7239aa8d5ff38
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text/plain
MetaboliChangesDuringRespiratory_2020.pdf.txt