2026-06-14T03:36:19Zhttps://repisalud.isciii.es/rest/oai/request

oai:repisalud.isciii.es:20.500.12105/94212025-05-23T13:08:32Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19609

00925njm 22002777a 4500 dc Martin-Vicente, Maria author González-Riaño, Carolina author Barbas, Coral author Jimenez-Sousa, Maria Angeles author Brochado-Kith, Oscar author Resino, Salvador author Martinez, Isidoro author 2020 Viral infections induce substantial metabolic changes in infected cells to optimize viral production while cells develop countermeasures to restrict that infection. Human respiratory syncytial virus (HRSV) is an infectious pathogen that causes severe lower respiratory tract infections (LRTI) in infants, the elderly, and immunocompromised adults for which no effective treatment or vaccine is currently available. In this study, variations in metabolite levels at different time points post-HRSV infection of epithelial cells were studied by untargeted metabolomics using liquid chromatography/mass spectrometry analysis of methanol cell extracts. Numerous metabolites were significantly upregulated after 18 hours post-infection, including nucleotides, amino acids, amino and nucleotide sugars, and metabolites of the central carbon pathway. In contrast, most lipid classes were downregulated. Additionally, increased levels of oxidized glutathione and polyamines were associated with oxidative stress in infected cells. These results show how HRSV infection influences cell metabolism to produce the energy and building blocks necessary for virus reproduction, suggesting potential therapeutic interventions against this virus. PLoS One. 2020 Mar 26;15(3):e0230844. 10.1371/journal.pone.0230844 1932-6203 1932-6203 PloS one 32214395 http://hdl.handle.net/20.500.12105/9421 Metabolic changes during respiratory syncytial virus infection of epithelial cells