2026-06-14T03:39:13Zhttps://repisalud.isciii.es/rest/oai/request

oai:repisalud.isciii.es:20.500.12105/94192024-09-27T21:28:14Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19609

00925njm 22002777a 4500 dc Muadica, Aly Salimo author Köster, Pamela Carolina author Dashti, Alejandro author Bailo-Barroso, Begoña author Hernandez-De-Mingo, Marta author Reh, Lucia author Balasegaram, Sooria author Verlander, Neville Q author Ruiz Chércoles, Esther author Carmena, David author 2020-03-25 Enteric parasites including Giardia duodenalis, Cryptosporidium spp., and to a lesser extent, Blastocystis sp. and Enterocytozoon bieneusi, are major worldwide contributors to diarrhoeal disease. Assessing their molecular frequency and diversity is important to ascertain the sources of infection, transmission dynamics, and zoonotic potential. Little molecular information is available on the genotypes of these pathogens circulating in apparently healthy children. Here, we show that asymptomatic carriage of G. duodenalis (17.4%, 95% CI: 15.5‒19.4%), Blastocystis sp. (13.0%, 95% CI: 11.4‒14.8%), and Cryptosporidium spp. (0.9%, 95% CI: 0.5‒1.5%) is common in children (1‒16 years; n = 1512) from Madrid, Spain. Our genotyping data indicate that; (i) the observed frequency and diversity of parasite genetic variants are very similar to those previously identified in Spanish clinical samples, so that the genotype alone does not predict the clinical outcome of the infection, (ii) anthroponotic transmission accounts for a large proportion of the detected cases, highlighting that good personal hygiene practices are important to minimizing the risk of infection, (iii) Blastocystis ST4 may represent a subtype of the parasite with higher pathogenic potential, and (iv) Enterocytozoon bieneusi does not represent a public health concern in healthy children. Microorganisms. 2020 Mar 25;8(4). pii: E466. 10.3390/microorganisms8040466 2076-2607 Microorganisms 32218318 http://hdl.handle.net/20.500.12105/9419 Blastocystis Cryptosporidium Enterocytozoon Giardia PCR Spain Asymptomatic children Genotyping Molecular detection Molecular epidemiology Molecular Diversity of Giardia duodenalis, Cryptosporidium spp. and Blastocystis sp. in Asymptomatic School Children in Leganés, Madrid (Spain)