2026-06-26T07:49:06Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/93742024-09-27T07:58:25Zcom_20.500.12105_19604com_20.500.12105_2051col_20.500.12105_19605
Repisalud
author
Fernandez-Messina, Lola
author
Rodriguez-Galan, Ana
author
de Yebenes, Virginia G
author
Gutierrez-Vazquez, Cristina
author
Tenreiro, Sandra
author
Seabra, Miguel C
author
Ramiro, Almudena R
author
Sanchez-Madrid, Francisco
funder
Ministerio de Economía y Competitividad (España)
funder
Comunidad de Madrid (España)
funder
Centro de Investigación Biomedica en Red - CIBER
funder
Instituto de Salud Carlos III
funder
Fundación La Marató TV3
funder
Fundación ProCNIC
funder
Fundación La Caixa
funder
Fundación Ramón Areces
funder
Fundación BBVA
funder
Ministerio de Ciencia, Innovación y Universidades (España)
2020-03-30T15:15:00Z
2020-03-30T15:15:00Z
2020-02-19
EMBO Rep. 2020:e48925
10.15252/embr.201948925
1469-3178
1469-221X
EMBO reports
32073750
http://hdl.handle.net/20.500.12105/9374
Intercellular communication orchestrates effective immune responses against disease-causing agents. Extracellular vesicles (EVs) are potent mediators of cell-cell communication. EVs carry bioactive molecules, including microRNAs, which modulate gene expression and function in the recipient cell. Here, we show that formation of cognate primary T-B lymphocyte immune contacts promotes transfer of a very restricted set of T-cell EV-microRNAs (mmu-miR20-a-5p, mmu-miR-25-3p, and mmu-miR-155-3p) to the B cell. Transferred EV-microRNAs target key genes that control B-cell function, including pro-apoptotic BIM and the cell cycle regulator PTEN. EV-microRNAs transferred during T-B cognate interactions also promote survival, proliferation, and antibody class switching. Using mouse chimeras with Rab27KO EV-deficient T cells, we demonstrate that the transfer of small EVs is required for germinal center reaction and antibody production in vivo, revealing a mechanism that controls B-cell responses via the transfer of EV-microRNAs of T-cell origin. These findings also provide mechanistic insight into the Griscelli syndrome, associated with a mutation in the Rab27a gene, and might explain antibody defects observed in this pathogenesis and other immune-related and inflammatory disorders.
eng
Antibody production
Exosomes
Extracellular vesicles
Germinal center (GC) reaction
microRNAs
Transfer of extracellular vesicle-microRNA controls germinal center reaction and antibody production
journal article
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URL
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TransferExtracellularVesicle-microRNA_2020.pdf
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text/plain
TransferExtracellularVesicle-microRNA_2020.pdf.txt