2026-06-26T07:49:31Zhttps://repisalud.isciii.es/rest/oai/request

oai:repisalud.isciii.es:20.500.12105/93742024-09-27T07:58:25Zcom_20.500.12105_19604com_20.500.12105_2051col_20.500.12105_19605

00925njm 22002777a 4500 dc Fernandez-Messina, Lola author Rodriguez-Galan, Ana author de Yebenes, Virginia G author Gutierrez-Vazquez, Cristina author Tenreiro, Sandra author Seabra, Miguel C author Ramiro, Almudena R author Sanchez-Madrid, Francisco author 2020-02-19 Intercellular communication orchestrates effective immune responses against disease-causing agents. Extracellular vesicles (EVs) are potent mediators of cell-cell communication. EVs carry bioactive molecules, including microRNAs, which modulate gene expression and function in the recipient cell. Here, we show that formation of cognate primary T-B lymphocyte immune contacts promotes transfer of a very restricted set of T-cell EV-microRNAs (mmu-miR20-a-5p, mmu-miR-25-3p, and mmu-miR-155-3p) to the B cell. Transferred EV-microRNAs target key genes that control B-cell function, including pro-apoptotic BIM and the cell cycle regulator PTEN. EV-microRNAs transferred during T-B cognate interactions also promote survival, proliferation, and antibody class switching. Using mouse chimeras with Rab27KO EV-deficient T cells, we demonstrate that the transfer of small EVs is required for germinal center reaction and antibody production in vivo, revealing a mechanism that controls B-cell responses via the transfer of EV-microRNAs of T-cell origin. These findings also provide mechanistic insight into the Griscelli syndrome, associated with a mutation in the Rab27a gene, and might explain antibody defects observed in this pathogenesis and other immune-related and inflammatory disorders. EMBO Rep. 2020:e48925 10.15252/embr.201948925 1469-3178 1469-221X EMBO reports 32073750 http://hdl.handle.net/20.500.12105/9374 Antibody production Exosomes Extracellular vesicles Germinal center (GC) reaction microRNAs Transfer of extracellular vesicle-microRNA controls germinal center reaction and antibody production