2026-06-14T03:28:50Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/93452024-11-29T14:46:00Zcom_20.500.12105_2173com_20.500.12105_2051col_20.500.12105_19597
Repisalud
author
Vela, Maria
author
Bueno, David
author
González-Navarro, Pablo
author
Brito, Ariadna
author
Fernandez, Lucia
author
Escudero, Adela
author
Valentín, Jaime
author
Mestre-Durán, Carmen
author
Arranz-Álvarez, Marina
author
Pérez de Diego, Rebeca
author
Mendiola, Marta
author
Pozo-Kreilinger, José Juan
author
Pérez-Martínez, Antonio
funder
Instituto de Salud Carlos III
funder
Asociación Española Contra el Cáncer
funder
CRIS contra el Cáncer
funder
Patients' Support Association Fundacion Mari Paz Jimenez Casado
funder
Fundación la Sonrisa de Alex para la investigación y el tratamiento del sarcoma de Ewing
2020-03-26T18:33:00Z
2020-03-26T18:33:00Z
2019-08-02
Front Immunol. 2019;10:1814.
10.3389/fimmu.2019.01814
1664-3224
1664-3224
Frontiers in immunology
31428099
http://hdl.handle.net/20.500.12105/9345
Sarcoma is one of the most severe forms of pediatric cancer and current therapies -chemotherapy and surgery- fail to eradicate the disease in half of patients. Preclinical studies combining new therapeutic approaches can be useful to design better therapies. On one hand, it is known that CXCR4 expression is implicated in rhabdomyosarcoma progression, so we analyzed relapses and chemotherapy-resistant rhabdomyosarcoma tumors from pediatric patients and found that they had particularly high levels of CXCR4 expression. Moreover, in assays in vitro, anti-CXCR4 blocking antibody (MDX1338) efficiently reduced migration and invasion of alveolar rhabdomyosarcoma RH30 cells. On the other hand, activated and expanded natural killer (NKAE) cell therapy showed high cytotoxicity against sarcoma cells in vitro and completely inhibited RH30 tumor implantation in vivo. Only the combination of MDX1338 and NKAE treatments completely suppressed metastasis in mice. In this study, we propose a novel therapeutic approach based on anti-CXCR4 blocking antibody in combination with NKAE cell therapy to prevent rhabdomyosarcoma tumor implantation and lung metastasis. These results provide the first evidence for the efficacy of this combined immunotherapy for preventing sarcoma disease dissemination.
eng
Activated and expanded natural killer (NKAE) cells
Chemokine C-X-C receptor 4 (CXCR4)
Immunotherapy
Metastasis
Sarcoma
Therapeutic antibody
Anti-CXCR4 Antibody Combined With Activated and Expanded Natural Killer Cells for Sarcoma Immunotherapy
journal article
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Data_Sheet_1_Anti-CXCR4 Antibody Combined With Activated and Expanded Natural Killer Cells for Sarcoma Immunotherapy.docx
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Anti-CXCR4AntibodyCombined_2019.pdf.txt
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