2026-06-14T03:45:06Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/93372024-09-27T21:10:00Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19609
Repisalud
author
Torrellas, Annhymariet
author
Ferrer, Elizabeth
author
Cruz, Israel
author
Lima, Héctor de
author
Delgado, Olinda
author
Rangel, José Carrero
author
Bravo, José Arturo
author
Chicharro, Carmen
author
Llanes-Acevedo, Ivonne Pamela
author
Miles, Michael A
author
Feliciangeli, María Dora
funder
Unión Europea. Comisión Europea
funder
Ministerio de Ciencia y Tecnología (España)
2020-03-25T17:41:04Z
2020-03-25T17:41:04Z
2018-12-06
Mem Inst Oswaldo Cruz. 2018 Dec 6;113(12):e180323.
10.1590/0074-02760180323
1678-8060
1678-8060
Memorias do Instituto Oswaldo Cruz
30540021
http://hdl.handle.net/20.500.12105/9337
BACKGROUND The transmission routes for American cutaneous leishmaniasis (ACL) are in flux, so studies examining its transmission in humans, mammalian hosts, and sand fly vectors are urgently needed. OBJECTIVES The aim of this work was understand the epidemiological cycles of Leishmania spp., which causes ACL in the Andean Region of Venezuela, by identifying the Leishmania and the sand fly species involved in human and dog infections. METHODS Thirty-one biopsies from patients in Mérida and Táchira states with suspected ACL were studied by both parasitological tests (cultures and hamster inoculation) and a molecular test [Internal transcribed spacer 1 (ITS1) nested polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP)]. We also conducted a survey to detect Leishmania infection in dogs (Immunifluorescence antibody test and ITS1 nested PCR-RFLP) and sand flies (ITS1 nested PCR-RFLP) from El Carrizal, a highly endemic focus of ACL in Venezuela. FINDINGS Three different Leishmania species were identified in the clinical samples from humans (Leishmania braziliensis, L. guyanensis, and L. mexicana) and dogs (L. guyanensis and L. mexicana). The predominant sand fly species found were those from the Verrucarum group (infected with L. mexicana) and Lutzomyia migonei (infected with L. guyanensis and L. mexicana). MAIN CONCLUSIONS We show that Lu. migonei may be the putative vector in two ACL epidemiological cycles, involving L. guyanensis and L. mexicana. We also report for the first time the presence of L. guyanensis in domestic animals.
eng
Molecular typing reveals the co-existence of two transmission cycles of American cutaneous leishmaniasis in the Andean Region of Venezuela with Lutzomyia migonei as the vector
journal article
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URL
https://repisalud.isciii.es/bitstreams/9545060e-923a-400f-ab2a-bf43b0d1713f/download
File
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MolecularTypingRevealsThe_2018.pdf
URL
https://repisalud.isciii.es/bitstreams/fc26899c-a8d2-4881-b31d-ae88b26314cc/download
File
MD5
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text/plain
MolecularTypingRevealsThe_2018.pdf.txt