2026-06-14T03:30:22Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/93282024-10-31T11:51:51Zcom_20.500.12105_19604com_20.500.12105_2051col_20.500.12105_19605
Repisalud
author
Lehmann, Guillermo L
author
Hanke-Gogokhia, Christin
author
Hu, Yang
author
Bareja, Rohan
author
Salfati, Zelda
author
Ginsberg, Michael
author
Nolan, Daniel J
author
Mendez-Huergo, Santiago P
author
Dalotto-Moreno, Tomas
author
Wojcinski, Alexandre
author
Ochoa, Francisca
author
Zeng, Shemin
author
Cerliani, Juan P
author
Panagis, Lampros
author
Zager, Patrick J
author
Mullins, Robert F
author
Ogura, Shuntaro
author
Lutty, Gerard A
author
Bang, Jakyung
author
Zippin, Jonathan H
author
Romano, Carmelo
author
Rabinovich, Gabriel A
author
Elemento, Olivier
author
Joyner, Alexandra L
author
Rafii, Shahin
author
Rodriguez-Boulan, Enrique
author
Benedicto, Ignacio
funder
National Institutes of Health (Estados Unidos)
funder
NIH - National Cancer Institute (NCI) (Estados Unidos)
funder
Comunidad de Madrid (España)
funder
Instituto de Salud Carlos III
funder
Ministerio de Ciencia, Innovación y Universidades (España)
funder
Fundación ProCNIC
funder
Agencia Nacional de Promoción Científica y Tecnológica (Argentina)
2020-03-25T12:37:10Z
2020-03-25T12:37:10Z
2020-06-01
J Exp Med. 2020; 217(6):e20190730
10.1084/jem.20190730
1540-9538
0022-1007
The Journal of experimental medicine
32196081
http://hdl.handle.net/20.500.12105/9328
The activity and survival of retinal photoreceptors depend on support functions performed by the retinal pigment epithelium (RPE) and on oxygen and nutrients delivered by blood vessels in the underlying choroid. By combining single-cell and bulk RNA sequencing, we categorized mouse RPE/choroid cell types and characterized the tissue-specific transcriptomic features of choroidal endothelial cells. We found that choroidal endothelium adjacent to the RPE expresses high levels of Indian Hedgehog and identified its downstream target as stromal GLI1+ mesenchymal stem cell-like cells. In vivo genetic impairment of Hedgehog signaling induced significant loss of choroidal mast cells, as well as an altered inflammatory response and exacerbated visual function defects after retinal damage. Our studies reveal the cellular and molecular landscape of adult RPE/choroid and uncover a Hedgehog-regulated choroidal immunomodulatory signaling circuit. These results open new avenues for the study and treatment of retinal vascular diseases and choroid-related inflammatory blinding disorders.
eng
Single-cell profiling reveals an endothelium-mediated immunomodulatory pathway in the eye choroid
journal article
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