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oai:repisalud.isciii.es:20.500.12105/92722024-09-27T20:48:26Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19609

00925njm 22002777a 4500 dc Botana, Laura author Matía, Belén author San Martín, Juan Víctor author Romero-Maté, Alberto author Castro, Alicia author Molina, Laura author Fernandez, Laura author Ibarra-Meneses, Ana Victoria author Aguado, José María author Sanchez Herrero, Carmen author Horrillo, Luis author Chicharro, Carmen author Nieto Martinez, Francisco Javier author Ortega, Sheila author Ruiz-Giardin, José Manuel author Carrillo, Eugenia author Moreno, Javier author 2018 Increased numbers of peripheral blood mononucleocytes (PBMC) and increased IFN-γ secretion following in vitro challenge of blood samples with soluble Leishmania antigen (SLA), have been proposed as biomarkers of specific cell-mediated immunity, indicating that treatment of visceral leishmaniasis (VL) has been successful. However, Leishmania infantum infection may manifest as cutaneous leishmaniasis (CL), and less commonly as localized leishmanial lymphadenopathy (LLL) or mucosal leishmaniasis (ML). The present work examines the value of these biomarkers as indicators of cured leishmaniasis presenting in these different forms. Blood samples were collected before and after treatment from patients living in Fuenlabrada (Madrid, Spain), an L. infantum-endemic area recently the center of a leishmaniasis outbreak. All samples were subjected to Leishmania-specific PCR, serological tests (IFAT and rK39-ICT), and the SLA-cell proliferation assay (SLA-CPA), recording PBMC proliferation and the associated changes in IFN-γ production. Differences in the results recorded for the active and cured conditions were only significant for VL. PCR returned positive results in 67% of patients with active VL and in 3% of those with cured leishmaniasis. Similarly, rK39-ICT returned a positive result in 77% of active VL samples vs. 52% in cured VL samples, and IFAT in 90% vs. 56%; in the SLA-CPA, PBMC proliferation was seen in 16% vs. 90%, and an associated increase in IFN-γ production of 14 and 84%, respectively. The present findings reinforce the idea that PBMC proliferation and increased IFN-γ production in SLA-stimulated PBMC provide biomarkers of clinical cure in VL. Other tests are urgently needed to distinguish between the cured and active forms of the other types of clinical leishmaniasis caused by L. infantum. Front Cell Infect Microbiol. 2018 Nov 13;8:381. 2235-2988 http://hdl.handle.net/20.500.12105/9272 30483480 10.3389/fcimb.2018.00381 2235-2988 Frontiers in cellular and infection microbiology IFN-γ Biomarker Cell proliferation assay Cure Cutaneous leishmaniasis Lymphadenopathic leishmaniasis Mucosal leishmaniasis Visceral leishmaniasis Cellular Markers of Active Disease and Cure in Different Forms of Leishmania infantum-Induced Disease