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oai:repisalud.isciii.es:20.500.12105/92622025-06-26T10:54:42Zcom_20.500.12105_2052com_20.500.12105_2051com_20.500.12105_14556com_20.500.12105_2337com_20.500.12105_2336col_20.500.12105_19609col_20.500.12105_14558col_20.500.12105_19616

00925njm 22002777a 4500 dc Gambera, Stefano author Abarrategi, Ander author Garcia-Castro, Javier author Morales-Molina, Alvaro author Roma, Josep author Alfranca, Arantzazu author Gonzalez-Camacho, Fernando author 2018 Osteosarcoma is a type of bone tumour characterized by considerable levels of phenotypic heterogeneity, aneuploidy, and a high mutational rate. The life expectancy of osteosarcoma patients has not changed during the last three decades and thus much remains to be learned about the disease biology. Here, we employ a RGB-based single-cell tracking system to study the clonal dynamics occurring in a de novo-induced murine osteosarcoma model. We show that osteosarcoma cells present initial polyclonal dynamics, followed by clonal dominance associated with adaptation to the microenvironment. Interestingly, the dominant clones are composed of subclones with a similar tumour generation potential when they are re-implanted in mice. Moreover, individual spontaneous metastases are clonal or oligoclonal, but they have a different cellular origin than the dominant clones present in primary tumours. In summary, we present evidence that osteosarcomagenesis can follow a neutral evolution model, in which different cancer clones coexist and propagate simultaneously. Nat Commun. 2018 Sep 28;9(1):3994. 10.1038/s41467-018-06401-z 2041-1723 2041-1723 Nature communications 30266933 http://hdl.handle.net/20.500.12105/9262 Clonal dynamics in osteosarcoma defined by RGB marking