2026-08-05T15:43:01Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/91722024-11-28T12:35:15Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19609col_20.500.12105_25179
Repisalud
author
Garcia-Rubio, Rocio
author
Monzon-Fernandez, Sara
author
Alcazar-Fuoli, Laura
author
Cuesta de la Plaza, Isabel
author
Mellado, Emilia
funder
Instituto de Salud Carlos III
funder
Ministerio de Economía, Industria y Competitividad (España)
funder
RETICS-Investigación en Patología Infecciosa (REIPI-ISCIII) (España)
funder
Unión Europea. Fondo Europeo de Desarrollo Regional (FEDER/ERDF)
2020-03-04T12:01:45Z
2020-03-04T12:01:45Z
2018-07-19
Genes (Basel). 2018 Jul 19;9(7). pii: E363.
10.3390/genes9070363
2073-4425
Genes
30029559
http://hdl.handle.net/20.500.12105/9172
Aspergillus fumigatus is a ubiquitous saprophytic mold and a major pathogen in immunocompromised patients. The effectiveness of triazole compounds, the A. fumigatus first line treatment, is being threatened by a rapid and global emergence of azole resistance. Whole genome sequencing (WGS) has emerged as an invaluable tool for the analysis of genetic differences between A. fumigatus strains, their genetic background, and antifungal resistance development. Although WGS analyses can provide a valuable amount of novel information, there are some limitations that should be considered. These analyses, based on genome-wide comparative data and single nucleotide variant (SNV) calling, are dependent on the quality of sequencing, assembling, the variant calling criteria, as well as on the suitable selection of the reference genome, which must be genetically close to the genomes included in the analysis. In this study, 28 A. fumigatus genomes sequenced in-house and 73 available in public data bases have been analyzed. All genomes were distributed in four clusters and showed a variable number of SNVs depending on the genome used as reference (Af293 or A1163). Each reference genome belonged to a different cluster. The results highlighted the importance of choosing the most suitable A. fumigatus reference genome to avoid misleading conclusions.
eng
Aspergillus fumigatus
Azole resistance
cyp51A
Reference genomes
Whole genome sequencing
Genome-Wide Comparative Analysis of Aspergillus fumigatus Strains: The Reference Genome as a Matter of Concern
research article
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URL
https://repisalud.isciii.es/bitstreams/de5553f7-2354-465a-877e-47e7448bd964/download
File
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application/pdf
GenomeWideComparativeAnalysis_2018.pdf
URL
https://repisalud.isciii.es/bitstreams/661f1b37-70b8-40e9-89c0-2c74f79dc80e/download
File
MD5
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text/plain
GenomeWideComparativeAnalysis_2018.pdf.txt