2026-06-15T11:34:45Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/91712025-05-29T11:20:01Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19609
Repisalud
author
Martin-Vicente, Maria
author
Gonzalez-Sanz, Ruben
author
Cuesta de la Plaza, Isabel
author
Monzon-Fernandez, Sara
author
Resino, Salvador
author
Martinez, Isidoro
funder
Comunidad de Madrid (España)
funder
Consejo Superior de Investigaciones Científicas (España)
2020-03-04T12:01:30Z
2020-03-04T12:01:30Z
2020-02-24
Vaccines (Basel). 2020 Feb 24;8(1). pii: E100.
10.3390/vaccines8010100
2076-393X
Vaccines
32102364
http://hdl.handle.net/20.500.12105/9171
Human respiratory syncytial virus (HRSV) causes severe lower respiratory tract infections in infants, the elderly, and immunocompromised adults. Regulation of the immune response against HRSV is crucial to limiting virus replication and immunopathology. The A20/TNFAIP3 protein is a negative regulator of nuclear factor kappa B (NF-kB) and interferon regulatory factors 3/7 (IRF3/7), which are key transcription factors involved in the inflammatory/antiviral response of epithelial cells to virus infection. Here, we investigated the impact of A20 downregulation or knockout on HRSV growth and the induction of the immune response in those cells. Cellular infections in which the expression of A20 was silenced by siRNAs or eliminated by gene knockout showed increased inflammatory/antiviral response and reduced virus production. Similar results were obtained when the expression of A20-interacting proteins, such as TAX1BP1 and ABIN1, was silenced. Additionally, downregulation of A20, TAX1BP1, and ABIN1 increased cell apoptosis in HRSV-infected cells. These results show that the downregulation of A20 expression might contribute in the control of HRSV infections by potentiating the early innate immune response and increasing apoptosis in infected cells.
eng
A20
Apoptosis
Innate immune response
Respiratory syncytial virus
Ubiquitination
Downregulation of A20 Expression Increases the Immune Response and Apoptosis and Reduces Virus Production in Cells Infected by the Human Respiratory Syncytial Virus
research article
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URL
https://repisalud.isciii.es/bitstreams/0ed956ad-8d4d-4f49-8934-0aa7dd1264c8/download
File
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1191106
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DownregulationOfA20Expression_2020.pdf
URL
https://repisalud.isciii.es/bitstreams/24568049-1917-457a-b8c2-8d74f714e9f4/download
File
MD5
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text/plain
DownregulationOfA20Expression_2020.pdf.txt