2026-06-14T03:31:39Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/91672024-09-27T21:24:26Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19609
Repisalud
author
Gomez Fontela, Miguel
author
Notario, Laura
author
Alari-Pahissa, Elisenda
author
Lorente, Elena
author
Lauzurica, Pilar
funder
Ministerio de Ciencia, Innovación y Universidades (España)
funder
Instituto de Salud Carlos III
funder
Ministerio de Educación, Cultura y Deporte (España)
2020-03-04T12:00:04Z
2020-03-04T12:00:04Z
2019
Genes (Basel). 2019 Aug 28;10(9). pii: E651.
10.3390/genes10090651
2073-4425
2073-4425
Genes
31466317
http://hdl.handle.net/20.500.12105/9167
The immune regulatory receptor CD69 is expressed upon activation in all types of leukocytes and is strongly regulated at the transcriptional level. We previously described that, in addition to the CD69 promoter, there are four conserved noncoding regions (CNS1-4) upstream of the CD69 promoter. Furthermore, we proposed that CNS2 is the main enhancer of CD69 transcription. In the present study, we mapped the transcription factor (TF) binding sites (TFBS) from ChIP-seq databases within CNS2. Through luciferase reporter assays, we defined a ~60 bp sequence that acts as the minimum enhancer core of mouse CNS2, which includes the Oct1 TFBS. This enhancer core establishes cooperative interactions with the 3' and 5' flanking regions, which contain RUNX1 BS. In agreement with the luciferase reporter data, the inhibition of RUNX1 and Oct1 TF expression by siRNA suggests that they synergistically enhance endogenous CD69 gene transcription. In summary, we describe an enhancer core containing RUNX1 and Oct1 BS that is important for the activity of the most potent CD69 gene transcription enhancer.
eng
CD69
Enhancer
Noncoding regions
Transcription factor
Transcriptional regulation
The Conserved Non-Coding Sequence 2 (CNS2) Enhances CD69 Transcription through Cooperation between the Transcription Factors Oct1 and RUNX1
journal article
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URL
https://repisalud.isciii.es/bitstreams/75bf5d74-bb24-4c2e-b779-8df7f6e4571c/download
File
MD5
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2227013
application/pdf
TheConservedNonCoding_2019.pdf
URL
https://repisalud.isciii.es/bitstreams/14ddf6d0-3398-4bfb-8258-197a0e18e4ba/download
File
MD5
d852182fd0ca7819784161869cf2a137
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text/plain
TheConservedNonCoding_2019.pdf.txt