2026-06-14T12:03:57Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/91552025-05-09T09:11:45Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19609
Repisalud
author
Vázquez-Morón, Sonia
author
Ryan, Pablo
author
Ardizone Jimenez, Beatriz
author
Martín, Dolores
author
Troya, Jesús
author
Cuevas, Guillermo
author
Valencia, Jorge
author
Jimenez-Sousa, Maria Angeles
author
Avellón, Ana
author
Resino, Salvador
funder
Instituto de Salud Carlos III
funder
Red de Investigación Cooperativa en Investigación en Sida (España)
2020-02-28T07:58:06Z
2020-02-28T07:58:06Z
2018
Sci Rep. 2018 Jan 30;8(1):1858.
10.1038/s41598-018-20312-5
2045-2322
2045-2322
Scientific reports
29382904
http://hdl.handle.net/20.500.12105/9155
Both hepatitis C virus (HCV) infection and human immunodeficiency virus (HIV) infection are underdiagnosed, particularly in low-income countries and in difficult-to-access populations. Our aim was to develop and evaluate a methodology for the detection of HCV and HIV infection based on capillary dry blood spot (DBS) samples taken under real-world conditions. We carried out a cross-sectional study of 139 individuals (31 healthy controls, 68 HCV-monoinfected patients, and 40 HCV/HIV-coinfected patients). ELISA was used for anti-HCV and anti-HIV antibody detection; and SYBR Green RT-PCR was used for HCV-RNA detection. The HIV serological analysis revealed 100% sensitivity, specificity, positive predictive value (PPV), and negative predictive value (NPV). The HCV serological analysis revealed a sensitivity of 92.6%, specificity of 100%, PPV of 100%, and NPV of 79.5%. Finally, the HCV-RNA detection test revealed a detection limit of 5 copies/µl with an efficiency of 100% and sensitivity of 99.1%, specificity of 100%, PPV of 100%, and NPV of 96.9%. In conclusion, our methodology was able to detect both HCV infection and HIV infection from the same DBS sample with good diagnostic performance. Screening for HCV and HIV using DBS might be a key strategy in the implementation of national programs for the control of both infections.
eng
Evaluation of dried blood spot samples for screening of hepatitis C and human immunodeficiency virus in a real-world setting
research article
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URL
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application/pdf
EvaluationOfDriedBlood_2018.pdf
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https://repisalud.isciii.es/bitstreams/62589e09-8c39-4d9d-b943-a449b17027b2/download
File
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text/plain
EvaluationOfDriedBlood_2018.pdf.txt