2026-06-14T05:07:06Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/91482025-06-13T09:51:02Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19617
Repisalud
author
Calero, Olga
author
Garcia-Albert, Luis
author
Rodríguez-Martín, Andrés
author
Veiga, Sergio
author
Calero, Miguel
2020-02-26T15:26:48Z
2020-02-26T15:26:48Z
2018
Sci Rep. 2018 Apr 13;8(1):5969.
10.1038/s41598-018-24320-3
2045-2322
2045-2322
Scientific reports
29654261
http://hdl.handle.net/20.500.12105/9148
Apolipoprotein E (apoE) is a 34 kDa glycoprotein involved in lipid metabolism. The human APOE gene encodes for three different apoE protein isoforms: E2, E3 and E4. The interest in apoE isoforms is high for epidemiological research, patient stratification and identification of those at increased risk for clinical trials and prevention. The isoform apoE4 is associated with increased risk for coronary heart and Alzheimer's diseases. This paper describes a method for specifically detecting the apoE4 isoform from biological fluids by taking advantage of the capacity of apoE to bind "specifically" to polystyrene surfaces as capture and a specific anti-apoE4 monoclonal antibody as reporter. Our results indicate that the apoE-polystyrene binding interaction is highly stable, resistant to detergents and acid and basic washes. The methodology here described is accurate, easily implementable, fast and cost-effective. Although at present, our technique is unable to discriminate homozygous APOE ε4/ε4 from APOE ε3/ε4 and ε2/ε4 heterozygous, it opens new avenues for the development of inexpensive, yet effective, tests for the detection of apoE4 for patients' stratification. Preliminary results indicated that this methodology is also adaptable into turbidimetric platforms, which make it a good candidate for clinical implementation through its translation to the clinical analysis routine.
eng
A fast and cost-effective method for apolipoprotein E isotyping as an alternative to APOE genotyping for patient screening and stratification
research article
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URL
https://repisalud.isciii.es/bitstreams/f5a85341-3d4e-4a9a-9f16-c6025812d883/download
File
MD5
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application/pdf
AFastAndCost_2018.pdf
URL
https://repisalud.isciii.es/bitstreams/10407b23-2951-4032-8ddf-5474264aa5c3/download
File
MD5
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text/plain
AFastAndCost_2018.pdf.txt