2026-06-15T11:35:02Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/91442025-05-30T13:00:35Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19617
Repisalud
author
Armenteros, Tomás
author
Andreu, Zoraida
author
Hortigüela, Rafael
author
Lie, D Chichung
author
Mira, Helena
funder
Ministerio de Economía y Competitividad (España)
2020-02-25T10:06:23Z
2020-02-25T10:06:23Z
2018
Sci Rep. 2018 Jun 18;8(1):9241.
10.1038/s41598-018-27581-0
2045-2322
2045-2322
Scientific reports
29915186
http://hdl.handle.net/20.500.12105/9144
Neuronal production from neural stem cells persists during adulthood in the subgranular zone of the hippocampal dentate gyrus. Extracellular signals provided by the hippocampal microenvironment regulate the neuronal fate commitment of the stem cell progeny. To date, the identity of those signals and their crosstalk has been only partially resolved. Here we show that adult rat hippocampal neural stem and progenitor cells (AH-NSPCs) express receptors for bone morphogenetic proteins (BMPs) and that the BMP/P-Smad pathway is active in AH-NSPCs undergoing differentiation towards the neuronal lineage. In vitro, exposure to the BMP2 and BMP4 ligands is sufficient to increase neurogenesis from AH-NSPCs in a WNT dependent manner while decreasing oligodendrogenesis. Moreover, BMP2/4 and WNT3A, a key regulator of adult hippocampal neurogenesis, cooperate to further enhance neuronal production. Our data point to a mechanistic convergence of the BMP and WNT pathways at the level of the T-cell factor/lymphoid enhancer factor gene Lef1. Altogether, we provide evidence that BMP signalling is an important regulator for the neuronal fate specification of AH-NSPCs cultures and we show that it significantly cooperates with the previously described master regulator of adult hippocampal neurogenesis, the WNT signalling pathway.
eng
BMP and WNT signalling cooperate through LEF1 in the neuronal specification of adult hippocampal neural stem and progenitor cells
research article
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URL
https://repisalud.isciii.es/bitstreams/139be96f-f1a8-47b6-9efe-73f1cfd7af53/download
File
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BMPAndWNTSignalling_2018.pdf
URL
https://repisalud.isciii.es/bitstreams/099080d4-05cb-40f7-a580-57e6938621b8/download
File
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text/plain
BMPAndWNTSignalling_2018.pdf.txt