2026-06-14T03:30:39Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/91322025-05-30T16:43:47Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19609
Repisalud
author
Garcia-Rodas, Rocio
author
Trevijano-Contador, Nuria
author
Román, Elvira
author
Janbon, Guilhem
author
Moyrand, Frédérique
author
Pla, Jesús
author
Casadevall, Arturo
author
Zaragoza, Oscar
funder
Ministerio de Economía y Competitividad (España)
2020-02-21T10:58:09Z
2020-02-21T10:58:09Z
2015
Front Microbiol. 2015 Aug 12;6:798.
10.3389/fmicb.2015.00798
1664-302X
Frontiers in microbiology
26322026
http://hdl.handle.net/20.500.12105/9132
Cryptococcus neoformans is an opportunistic fungal pathogen that has several well-described virulence determinants. A polysaccharide capsule and the ability to produce melanin are among the most important. Melanization occurs both in vitro, in the presence of catecholamine and indole compounds, and in vivo during the infection. Despite the importance of melanin production for cryptococcal virulence, the component and mechanisms involved in its synthesis have not been fully elucidated. In this work, we describe the role of a G1/S cyclin (Cln1) in the melanization process. Cln1 has evolved specifically with proteins present only in other basidiomycetes. We found that Cln1 is required for the cell wall stability and production of melanin in C. neoformans. Absence of melanization correlated with a defect in the expression of the LAC1 gene. The relation between cell cycle elements and melanization was confirmed by the effect of drugs that cause cell cycle arrest at a specific phase, such as rapamycin. The cln1 mutant was consistently more susceptible to oxidative damage in a medium that induces melanization. Our results strongly suggest a novel and hitherto unrecognized role for C. neoformans Cln1 in the expression of virulence traits.
eng
Cryptococcus CLN1
Cell wall
Laccase
Melanin
Role of Cln1 during melanization of Cryptococcus neoformans
research article
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RoleOfCln1During_2015.pdf
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RoleOfCln1During_2015.pdf.txt