2026-06-14T05:05:38Zhttps://repisalud.isciii.es/rest/oai/request

oai:repisalud.isciii.es:20.500.12105/91322025-05-30T16:43:47Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19609

00925njm 22002777a 4500 dc Garcia-Rodas, Rocio author Trevijano-Contador, Nuria author Román, Elvira author Janbon, Guilhem author Moyrand, Frédérique author Pla, Jesús author Casadevall, Arturo author Zaragoza, Oscar author 2015 Cryptococcus neoformans is an opportunistic fungal pathogen that has several well-described virulence determinants. A polysaccharide capsule and the ability to produce melanin are among the most important. Melanization occurs both in vitro, in the presence of catecholamine and indole compounds, and in vivo during the infection. Despite the importance of melanin production for cryptococcal virulence, the component and mechanisms involved in its synthesis have not been fully elucidated. In this work, we describe the role of a G1/S cyclin (Cln1) in the melanization process. Cln1 has evolved specifically with proteins present only in other basidiomycetes. We found that Cln1 is required for the cell wall stability and production of melanin in C. neoformans. Absence of melanization correlated with a defect in the expression of the LAC1 gene. The relation between cell cycle elements and melanization was confirmed by the effect of drugs that cause cell cycle arrest at a specific phase, such as rapamycin. The cln1 mutant was consistently more susceptible to oxidative damage in a medium that induces melanization. Our results strongly suggest a novel and hitherto unrecognized role for C. neoformans Cln1 in the expression of virulence traits. Front Microbiol. 2015 Aug 12;6:798. 10.3389/fmicb.2015.00798 1664-302X Frontiers in microbiology 26322026 http://hdl.handle.net/20.500.12105/9132 Cryptococcus CLN1 Cell wall Laccase Melanin Role of Cln1 during melanization of Cryptococcus neoformans