2026-04-27T01:59:14Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/91092024-09-27T21:58:46Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19609
Repisalud
author
García, Nerea
author
Hernández, Marta
author
Gutierrez-Boada, Maialen
author
Valero, Antonio
author
Navarro, Alejandro
author
Muñoz-Chimeno, Milagros
author
Fernández-Manzano, Alvaro
author
Escobar, Franco Matías
author
Martínez, Irene
author
Bárcena, Carmen
author
González, Sergio
author
Avellón, Ana
author
Eiros, José María
author
Fongaro, Gislaine
author
Domínguez, Lucas
author
Goyache, Joaquín
author
Rodríguez-Lázaro, David
funder
Ministerio de Ciencia e Innovación (España)
2020-02-19T08:14:52Z
2020-02-19T08:14:52Z
2020
Front Microbiol. 2020 Jan 28;10:2990.
1664-302X
http://hdl.handle.net/20.500.12105/9109
32047480
10.3389/fmicb.2019.02990
Frontiers in microbiology
Zoonotic hepatitis E, mainly caused by hepatitis E virus (HEV) genotype (gt) 3, is a foodborne disease that has emerged in Europe in recent decades. The main animal reservoir for genotype 3 is domestic pigs. Pig liver and liver derivates are considered the major risk products, and studies focused on the presence of HEV in pig muscles are scarce. The objective of the present study was to evaluate the presence of HEV in different organs and tissues of 45 apparently healthy pigs from nine Spanish slaughterhouses (50% national production) that could enter into the food supply chain. Anti-HEV antibodies were evaluated in serum by an ELISA test. Ten samples from each animal were analyzed for the presence of HEV RNA by reverse transcription real-time PCR (RT-qPCR). The overall seroprevalence obtained was 73.3% (33/45). From the 450 samples analyzed, a total of 26 RT-qPCR positive samples were identified in the liver (7/45), feces (6/45), kidney (5/45), heart (4/45), serum (3/45), and diaphragm (1/45). This is the first report on detection of HEV RNA in kidney and heart samples of naturally infected pigs. HEV RNA detection was negative for rib, bacon, lean ham, and loin samples. These findings indicate that pig meat could be considered as a low risk material for foodborne HEV infection.
eng
Food safety
Hepatitis E virus
Pig organs
Pigs
Pork cuttings
Prevalence
Seroprevalence
Slaughterhouse
Occurrence of Hepatitis E Virus in Pigs and Pork Cuts and Organs at the Time of Slaughter, Spain, 2017
journal article
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OccurrenceOfHepatitisE_2020.pdf
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OccurrenceOfHepatitisE_2020.pdf.txt