2026-04-27T01:59:09Zhttps://repisalud.isciii.es/rest/oai/request

oai:repisalud.isciii.es:20.500.12105/91092024-09-27T21:58:46Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19609

00925njm 22002777a 4500 dc García, Nerea author Hernández, Marta author Gutierrez-Boada, Maialen author Valero, Antonio author Navarro, Alejandro author Muñoz-Chimeno, Milagros author Fernández-Manzano, Alvaro author Escobar, Franco Matías author Martínez, Irene author Bárcena, Carmen author González, Sergio author Avellón, Ana author Eiros, José María author Fongaro, Gislaine author Domínguez, Lucas author Goyache, Joaquín author Rodríguez-Lázaro, David author 2020 Zoonotic hepatitis E, mainly caused by hepatitis E virus (HEV) genotype (gt) 3, is a foodborne disease that has emerged in Europe in recent decades. The main animal reservoir for genotype 3 is domestic pigs. Pig liver and liver derivates are considered the major risk products, and studies focused on the presence of HEV in pig muscles are scarce. The objective of the present study was to evaluate the presence of HEV in different organs and tissues of 45 apparently healthy pigs from nine Spanish slaughterhouses (50% national production) that could enter into the food supply chain. Anti-HEV antibodies were evaluated in serum by an ELISA test. Ten samples from each animal were analyzed for the presence of HEV RNA by reverse transcription real-time PCR (RT-qPCR). The overall seroprevalence obtained was 73.3% (33/45). From the 450 samples analyzed, a total of 26 RT-qPCR positive samples were identified in the liver (7/45), feces (6/45), kidney (5/45), heart (4/45), serum (3/45), and diaphragm (1/45). This is the first report on detection of HEV RNA in kidney and heart samples of naturally infected pigs. HEV RNA detection was negative for rib, bacon, lean ham, and loin samples. These findings indicate that pig meat could be considered as a low risk material for foodborne HEV infection. Front Microbiol. 2020 Jan 28;10:2990. 1664-302X http://hdl.handle.net/20.500.12105/9109 32047480 10.3389/fmicb.2019.02990 Frontiers in microbiology Food safety Hepatitis E virus Pig organs Pigs Pork cuttings Prevalence Seroprevalence Slaughterhouse Occurrence of Hepatitis E Virus in Pigs and Pork Cuts and Organs at the Time of Slaughter, Spain, 2017