2026-08-29T12:39:37Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/90962024-09-27T21:55:25Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19609
Repisalud
author
Caramalho, Rita
author
Madl, Lisa
author
Rosam, Katharina
author
Rambach, Günter
author
Speth, Cornelia
author
Pallua, Johannes
author
Larentis, Thomas
author
Araújo, Ricardo
author
Alastruey-Izquierdo, Ana
author
Lass-Flörl, Cornelia
author
Lackner, Michaela
2020-02-14T11:47:28Z
2020-02-14T11:47:28Z
2019-10-11
J Fungi (Basel). 2019 Oct 11;5(4). pii: E98.
10.3390/jof5040098
2309-608X
2309-608X
Journal of fungi (Basel, Switzerland)
31614610
http://hdl.handle.net/20.500.12105/9096
Mucormycosis infections are infrequent yet aggressive and serious fungal infections. Early diagnosis of mucormycosis and its discrimination from other fungal infections is required for targeted treatment and more favorable patient outcomes. The majority of the molecular assays use 18 S rDNA. In the current study, we aimed to explore the potential of the mitochondrial rnl (encoding for large-subunit-ribosomal-RNA) gene as a novel molecular marker suitable for research and diagnostics. Rnl was evaluated as a marker for: (1) the Mucorales family, (2) species identification (Rhizopus arrhizus, R. microsporus, Mucor circinelloides, and Lichtheimia species complexes), (3) growth stage, and (4) quantification. Sensitivity, specificity, discriminatory power, the limit of detection (LoD), and cross-reactivity were evaluated. Assays were tested using pure cultures, spiked clinical samples, murine organs, and human paraffin-embedded-tissue (FFPE) samples. Mitochondrial markers were found to be superior to nuclear markers for degraded samples. Rnl outperformed the UMD universal® (Molyzm) marker in FFPE (71.5% positive samples versus 50%). Spiked blood samples highlighted the potential of rnl as a pan-Mucorales screening test. Fungal burden was reproducibly quantified in murine organs using standard curves. Identification of pure cultures gave a perfect (100%) correlation with the detected internal transcribed spacer (ITS) sequence. In conclusion, mitochondrial genes, such as rnl, provide an alternative to the nuclear 18 S rDNA genes and deserve further evaluation.
eng
HRM
Activity marker
Growth stage
Large subunit ribosomal DNA
Mitochondrial genes
Mucormycosis
Pan-Mucorales
qPCR
Seminested PCR
Evaluation of a Novel Mitochondrial Pan-Mucorales Marker for the Detection, Identification, Quantification, and Growth Stage Determination of Mucormycetes
journal article
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EvaluationOfANovel_2019.pdf
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EvaluationOfANovel_2019.pdf.txt