2026-06-14T03:46:58Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/90912025-06-13T09:09:08Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19617
Repisalud
author
López-Tobón, Alejandro
author
Villa, Carlo Emanuele
author
Cheroni, Cristina
author
Trattaro, Sebastiano
author
Caporale, Nicolò
author
Conforti, Paola
author
Iennaco, Raffaele
author
Lachgar-Ruiz, Maria
author
Rigoli, Marco Tullio
author
Marcó de la Cruz, Berta
author
Lo Riso, Pietro
author
Tenderini, Erika
author
Troglio, Flavia
author
De Simone, Marco
author
Liste-Noya, Isabel
author
Macino, Giuseppe
author
Pagani, Massimiliano
author
Cattaneo, Elena
author
Testa, Giuseppe
funder
Italian Association for Cancer Research
funder
Consiglio Nazionale delle Ricerche (Italia)
funder
Fondazione Cariplo
funder
Unión Europea. Comisión Europea. European Research Council (ERC)
2020-02-13T15:09:51Z
2020-02-13T15:09:51Z
2019-11-12
Stem Cell Reports. 2019 Nov 12;13(5):847-861.
10.1016/j.stemcr.2019.09.005
2213-6711
2213-6711
Stem cell reports
31607568
http://hdl.handle.net/20.500.12105/9091
The regulation of the proliferation and polarity of neural progenitors is crucial for the development of the brain cortex. Animal studies have implicated glycogen synthase kinase 3 (GSK3) as a pivotal regulator of both proliferation and polarity, yet the functional relevance of its signaling for the unique features of human corticogenesis remains to be elucidated. We harnessed human cortical brain organoids to probe the longitudinal impact of GSK3 inhibition through multiple developmental stages. Chronic GSK3 inhibition increased the proliferation of neural progenitors and caused massive derangement of cortical tissue architecture. Single-cell transcriptome profiling revealed a direct impact on early neurogenesis and uncovered a selective role of GSK3 in the regulation of glutamatergic lineages and outer radial glia output. Our dissection of the GSK3-dependent transcriptional network in human corticogenesis underscores the robustness of the programs determining neuronal identity independent of tissue architecture.
eng
GSK3
Corticogenesis
Human brain organoids
Outer radial glia
Single cell transcriptomics
Human Cortical Organoids Expose a Differential Function of GSK3 on Cortical Neurogenesis
research article
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URL
https://repisalud.isciii.es/bitstreams/77ce10be-108d-434f-a0d8-80e944d39b69/download
File
MD5
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application/pdf
HumanCorticalOrganoidsExpose_2019.pdf
URL
https://repisalud.isciii.es/bitstreams/6b9cf94f-98c8-4717-bbad-f8b7ee052466/download
File
MD5
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text/plain
HumanCorticalOrganoidsExpose_2019.pdf.txt