2026-06-14T03:54:08Zhttps://repisalud.isciii.es/rest/oai/request

oai:repisalud.isciii.es:20.500.12105/90912025-06-13T09:09:08Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19617

00925njm 22002777a 4500 dc López-Tobón, Alejandro author Villa, Carlo Emanuele author Cheroni, Cristina author Trattaro, Sebastiano author Caporale, Nicolò author Conforti, Paola author Iennaco, Raffaele author Lachgar-Ruiz, Maria author Rigoli, Marco Tullio author Marcó de la Cruz, Berta author Lo Riso, Pietro author Tenderini, Erika author Troglio, Flavia author De Simone, Marco author Liste-Noya, Isabel author Macino, Giuseppe author Pagani, Massimiliano author Cattaneo, Elena author Testa, Giuseppe author 2019-11-12 The regulation of the proliferation and polarity of neural progenitors is crucial for the development of the brain cortex. Animal studies have implicated glycogen synthase kinase 3 (GSK3) as a pivotal regulator of both proliferation and polarity, yet the functional relevance of its signaling for the unique features of human corticogenesis remains to be elucidated. We harnessed human cortical brain organoids to probe the longitudinal impact of GSK3 inhibition through multiple developmental stages. Chronic GSK3 inhibition increased the proliferation of neural progenitors and caused massive derangement of cortical tissue architecture. Single-cell transcriptome profiling revealed a direct impact on early neurogenesis and uncovered a selective role of GSK3 in the regulation of glutamatergic lineages and outer radial glia output. Our dissection of the GSK3-dependent transcriptional network in human corticogenesis underscores the robustness of the programs determining neuronal identity independent of tissue architecture. Stem Cell Reports. 2019 Nov 12;13(5):847-861. 10.1016/j.stemcr.2019.09.005 2213-6711 2213-6711 Stem cell reports 31607568 http://hdl.handle.net/20.500.12105/9091 GSK3 Corticogenesis Human brain organoids Outer radial glia Single cell transcriptomics Human Cortical Organoids Expose a Differential Function of GSK3 on Cortical Neurogenesis